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Biology LibreTexts

7.4: Materials and Procedures

  • Page ID
    40193
  • Materials

    Wet Mount Materials:

    • Glass slides
    • Coverslips
    • Lactophenol Cotton Blue Stain

    Live materials:

    • Your environmental plates-DO NOT OPEN
    • 1 SDA plate of Saccharomyces cerevisiae
    • Penicillium notatum – on a plate-demo only, DO NOT OPEN
    • Rhizopus stolonifer – on a plate -demo only, DO NOT OPEN

    Preserved Materials:

    • Amanita
    • Other mushrooms- Morchella, Puffball, Bird Nest Fungus
    • Spore print
    • Fungal pathogens- Black knot fungus, Corn smut
    • Prepared Slides:
    • Rhizopus, Penicillium, Aspergillus (mixed types—all three on one slide)
    • Rhizopus zygospore
    • Coccidioides immitis
    • Candida albicans (yeast)
    • Coprinus basidiospores
    • Pezizza ascospores

    Procedures

    1. Live specimens:
      1. Wet Mount of Saccharomyces cerevisiae:
        1. Place one drop of Lactophenol Cotton Blue onto a cleaned slide.
        2. Aseptically pick a small amount of growth, with a loop, from the SDA plate and transfer it to the drop of Lactophenol Cotton Blue; mix well and carefully place a coverslip over the stain.
        3. Observe under the microscope (low and high power) with bright-field, dark-field, and phase contrast. Draw some of your observations. Look for areas with well-separated cells and for cells that are budding. Keep your eyes on the budding cells and you may see the daughter cells separate!
      2. Observe under the dissecting scope and then draw the live plates of Penicillium notatum and Rhizopus stolonifer (DO NOT OPEN the plates). Observe the top and the reverse. Note structure/texture, color, etc.
    2. Preserved specimens: Observe and draw.
    3. Prepared slides: Observe, draw, label, and measure the fungi and their structures. Refer to images provided in order to help you find the correct organism/structure.

    Results

    1. Draw the yeast, plates, and prepared slides. Draw the asexual spores (mixed type slide) of each fungi separately.
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