7.4: Materials and Procedures
- Page ID
- 40193
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Wet Mount Materials:
- Glass slides
- Coverslips
- Lactophenol Cotton Blue Stain
Live materials:
- Your environmental plates-DO NOT OPEN
- 1 SDA plate of Saccharomyces cerevisiae
- Penicillium notatum – on a plate-demo only, DO NOT OPEN
- Rhizopus stolonifer – on a plate -demo only, DO NOT OPEN
Preserved Materials:
- Amanita
- Other mushrooms- Morchella, Puffball, Bird Nest Fungus
- Spore print
- Fungal pathogens- Black knot fungus, Corn smut
- Prepared Slides:
- Rhizopus, Penicillium, Aspergillus (mixed types—all three on one slide)
- Rhizopus zygospore
- Coccidioides immitis
- Candida albicans (yeast)
- Coprinus basidiospores
- Pezizza ascospores
Procedures
- Live specimens:
- Wet Mount of Saccharomyces cerevisiae:
- Place one drop of Lactophenol Cotton Blue onto a cleaned slide.
- Aseptically pick a small amount of growth, with a loop, from the SDA plate and transfer it to the drop of Lactophenol Cotton Blue; mix well and carefully place a coverslip over the stain.
- Observe under the microscope (low and high power) with bright-field, dark-field, and phase contrast. Draw some of your observations. Look for areas with well-separated cells and for cells that are budding. Keep your eyes on the budding cells and you may see the daughter cells separate!
- Observe under the dissecting scope and then draw the live plates of Penicillium notatum and Rhizopus stolonifer (DO NOT OPEN the plates). Observe the top and the reverse. Note structure/texture, color, etc.
- Wet Mount of Saccharomyces cerevisiae:
- Preserved specimens: Observe and draw.
- Prepared slides: Observe, draw, label, and measure the fungi and their structures. Refer to images provided in order to help you find the correct organism/structure.
Results
- Draw the yeast, plates, and prepared slides. Draw the asexual spores (mixed type slide) of each fungi separately.
