14.4: Materials and Procedures
- Page ID
- 40242
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Materials
- 3 FTM tubes
- 2 TSA plates
- GasPak jar, sachets, indicator
- Bacterial cultures:
- Staphylococcus saprophyticus (Use student stock organism)
- Pseudomonas fluorescens (Use student stock organism)
- Clostridium sporogenes (Use slant on front table, one per table only)
Procedures
- Quickly streak each of the 3 organisms onto 2 TSA plates:
- Immediately place 1 plate in the anaerobe jar. Incubate the other plate aerobically in your class incubator.
- Inoculate each organism into a separate Fluid Thioglycolate Medium(FTM):
Staphylococcus saprophyticus, Pseudomonas fluorescens, Clostridium sporogenes
- Incubate FTM tubes in your class incubator.
Results
- GasPak: Compare the GasPak plate to the plate incubated aerobically and fill in the following chart.
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Organism |
GasPak growth (+/-) |
Aerobic growth (+/-) |
Oxygen requirement type |
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- FTM: Draw your FTM tubes and label each with the appropriate oxygen requirement type
Contributors and Attributions
Kelly C. Burke (College of the Canyons)