3.1.4: DNA Extraction Supplies
- Page ID
- 67611
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DNA Extraction:
While many vendors offer mini-prep kits that will work for this step, Zymo provides an excellent kit (Cat#: D4210) with great results at a reasonable price.
|
Materials |
Amount Needed (Per Reaction) |
|
ZymoPURE™ P1 (Red) |
250 uL |
|
ZymoPURE™ P2 (Green) |
250 uL |
|
ZymoPURE™ P3 (Yellow) |
250 uL |
|
ZymoPURE™ Binding Buffer |
275 uL |
|
ZymoPURE™ Wash 1 |
800 uL |
|
ZymoPURE™ Wash 2 |
1000 uL |
|
ZymoPURE™ Elution Buffer |
25 uL |
|
Zymo-Spin™ II-P Columns (must have purple ring) |
1 |
|
Collection Tubes |
1 |
|
Serological Pipets |
2 (at least 2 mL) |
|
Microcentrifuge Tubes |
3 |
|
Centrifuge |
1 (used by class) |
|
Micropipettes and Tips |
100-1000 uL, 10-100 uL, 1-10 uL |
|
Ice Bucket |
1 |
|
UV Spectrophotometer |
1 (used by class) |