3.1.2: Adding Antibiotics
- Page ID
- 67609
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*Color coding LB-agar plates is recommended to simplify which plate contains which antibiotic*
**Addition of antibiotics to media is done after autoclaving, when the media has cooled to 50-55oC**
Ampicillin (Amp/A): *Working concentration 100 ug/mL freezer stock 100 mg/mL (in water)
- Add 1 mL of 100 mg/mL ampicillin (dissolved in water) into 1 L of LB broth or agar
Chloramphenicol (Chlor/C): *Working concentration 35 ug/mL freezer stock 35 mg/mL (in EtOH)
- Add 1 mL of 35 mg/mL chloramphenicol (dissolved in ethanol) into 1 L of LB broth or agar
Kanamycin (Kan/K): *Working Concentration 50 ug/mL freezer stock 50 mg/mL (in water)
- Add 1 mL of 50 mg/mL kanamycin (dissolved in water) into 1 L of LB broth or agar
Tetracycline (Tet/T): *Working concentration 10 ug/mL freezer stock 10 mg/mL (in water)
- Add 1 mL of 10 mg/mL tetracycline (dissolved in water) into 1 L LB broth or agar