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- https://bio.libretexts.org/Learning_Objects/Laboratory_Experiments/Microbiology_Labs/Microbiology_for_Allied_Health_Students%3A_Lab_Manual/3.04%3A_CatalaseCatalase is an enzyme that breaks hydrogen peroxide into water and oxygen. Hydrogen peroxide is a common byproduct of metabolic reactions occurring in an environment where water and oxygen are present...Catalase is an enzyme that breaks hydrogen peroxide into water and oxygen. Hydrogen peroxide is a common byproduct of metabolic reactions occurring in an environment where water and oxygen are present, but it is toxic to cells. Therefore, most organisms that survive in the presence of oxygen contain enzymes to degrade the hydrogen peroxide.
- https://bio.libretexts.org/Learning_Objects/Laboratory_Experiments/Microbiology_Labs/Book%3A_General_Microbiology_Lab_Manual_(Pakpour_and_Horgan)/Lab_11%3A_Biochemical_Tests_(Day_2)The final step in the chain may involve the use of the enzyme cytochrome oxidase, which catalyzes the oxidation of cytochrome c while reducing oxygen to form water. It contains peptone, phenol red (a ...The final step in the chain may involve the use of the enzyme cytochrome oxidase, which catalyzes the oxidation of cytochrome c while reducing oxygen to form water. It contains peptone, phenol red (a pH indicator), one carbohydrate (i.e glucose or lactose), and a Durham tube to test for the ability to convert the end product of glycolysis, pyruvic acid, into CO2 gas. If the urea in the broth is degraded and ammonia is produced, the pH rises and the media turns pink.
- https://bio.libretexts.org/Learning_Objects/Laboratory_Experiments/Microbiology_Labs/Microbiology_Labs_I/26%3A_Catalase_TestHydrogen peroxide H₂O₂ is a by-product of respiration and is lethal if it accumulates in the cell. The enzyme catalase degrades the hydrogen peroxide in the cell before it can do any cell damage. It ...Hydrogen peroxide H₂O₂ is a by-product of respiration and is lethal if it accumulates in the cell. The enzyme catalase degrades the hydrogen peroxide in the cell before it can do any cell damage. It splits the H₂O₂ to free oxygen (bubbles) and water. Generally, the test reaction is very fast and obvious bubbles will be seen. Catalase is made by your own cells, as well as a variety of other cells, including many bacteria. This test is particularly important for the gram+ bacteria.
- https://bio.libretexts.org/Bookshelves/Microbiology/Microbiology_Laboratory_Manual_(Miller)/01%3A_Labs/1.22%3A_Catalase_TestFlavoproteins, usually the initial electron carriers in the ETS, can transfer electrons directly to O 2 bypassing the rest of the ETS. In the last step of the ETS, an incomplete reduction can produce ...Flavoproteins, usually the initial electron carriers in the ETS, can transfer electrons directly to O 2 bypassing the rest of the ETS. In the last step of the ETS, an incomplete reduction can produce the superoxide radical O – which is then converted to H 2 O 2 by the enzyme superoxide dismutase. If the hydrogen peroxide makes contact with the metal, you could see bubbles because of the interaction of the metal and the H 2 O 2 . This may produce a false positive result.
- https://bio.libretexts.org/Workbench/Clinical_Microbiology_Lab_Manual/09%3A_Biochemical_Tests_(Day_2)The presence of clear halos surrounding colonies is positive for their ability to digest the starch and indicates the presence of alpha-amylase. The final step in the chain may involve the use of the ...The presence of clear halos surrounding colonies is positive for their ability to digest the starch and indicates the presence of alpha-amylase. The final step in the chain may involve the use of the enzyme cytochrome oxidase, which catalyzes the oxidation of cytochrome c while reducing oxygen to form water. How did the results observed on the MSA and EMB agar correlate to the Gram reaction of the bacteria?
- https://bio.libretexts.org/Bookshelves/Introductory_and_General_Biology/Unfolding_the_Mystery_of_Life_-_Biology_Lab_Manual_for_Non-Science_Majors_(Genovesi_Blinderman_and_Natale)/06%3A_Enzymes/6.02%3A_CatalaseWhen hydrogen peroxide comes in contact with the cut, it reacts with the catalase enzyme in the damaged cells to produce oxygen foam. The height of the foam is an indication of the amount of catalase ...When hydrogen peroxide comes in contact with the cut, it reacts with the catalase enzyme in the damaged cells to produce oxygen foam. The height of the foam is an indication of the amount of catalase activity present. State a hypothesis concerning the relative abilities of the various tissue types to catalyze the conversion of hydrogen peroxide to water and oxygen. Add the appropriate extract to each tube to the level of the 1 cm line.
