Search
- Filter Results
- Location
- Classification
- Include attachments
- https://bio.libretexts.org/Learning_Objects/Laboratory_Experiments/Microbiology_Labs/Microbiology_Labs_I/40%3A__Decarboxylation_of_Amino_AcidsThere are 3 decarboxylase enzymes we can test for--arginine decarboxylase, ornithine decarboxylase, and lysine decarboxylase. These enzymes break the bond holding the carboxylic (-COOH) group to the r...There are 3 decarboxylase enzymes we can test for--arginine decarboxylase, ornithine decarboxylase, and lysine decarboxylase. These enzymes break the bond holding the carboxylic (-COOH) group to the rest of the amino acid. As a result, the end product is a basic chemical which causes the pH to go up, changing the indicator brom cresol purple to turn purple.
- https://bio.libretexts.org/Learning_Objects/Laboratory_Experiments/Microbiology_Labs/Microbiology_Labs_I/22A%3A_Identification_of_Staphylococcus_SpeciesOf the bacteria which can grow in the presence of high NaCl, some are halophilic (requiring a certain concentration of salt to grow) while other are haloduric (do not use the salt, but can tolerate it...Of the bacteria which can grow in the presence of high NaCl, some are halophilic (requiring a certain concentration of salt to grow) while other are haloduric (do not use the salt, but can tolerate it). It can be a complete breakdown of the cells, with the release of hemoglobin and a clearing of the red from the surrounding medium around the colony.
- https://bio.libretexts.org/Learning_Objects/Laboratory_Experiments/Microbiology_Labs/Microbiology_Labs_I/22C%3A_Flowchart_for_Bacterial_UnknownLearning Objectives To use the flowchart as a guide to find bacterial unknown genus Flowchart to find unknown bacterial genus Contributors and Attributions Jackie Reynolds, Professor of Biology (Richl...Learning Objectives To use the flowchart as a guide to find bacterial unknown genus Flowchart to find unknown bacterial genus Contributors and Attributions Jackie Reynolds, Professor of Biology (Richland College)
- https://bio.libretexts.org/Learning_Objects/Laboratory_Experiments/Microbiology_Labs/Microbiology_Labs_I/00%3A_Front_Matter
- https://bio.libretexts.org/Learning_Objects/Laboratory_Experiments/Microbiology_Labs/Microbiology_Labs_I/03%3A_Dilution_Techniques_and_PipettingRemember that the reading is taken at the bottom of the meniscus. (the reading on the pipet in picture is at 8.1ml. Dilution set 1: Transfer 0.5ml of blue water into the 4.5ml of water, then 1ml of tu...Remember that the reading is taken at the bottom of the meniscus. (the reading on the pipet in picture is at 8.1ml. Dilution set 1: Transfer 0.5ml of blue water into the 4.5ml of water, then 1ml of tube 1 into the next tube of 9ml water. Dilution set 2: Transfer 1ml of blue water into the 4ml of water, then 0.5ml of tube 1 into the next tube of 9.5ml. Dilution set 3: Transfer 1ml of blue water into the 3ml of water, then 0.5ml of tube 1 into the next tube of 12ml.
- https://bio.libretexts.org/Learning_Objects/Laboratory_Experiments/Microbiology_Labs/Microbiology_Labs_I/31%3A_Coagulase_TestThe enzyme coagulase, produced by a few of the Staphylococcus species, is a key feature of pathogenic Staph. The enzyme produces coagulation of blood, allowing the organism to "wall " its infection of...The enzyme coagulase, produced by a few of the Staphylococcus species, is a key feature of pathogenic Staph. The enzyme produces coagulation of blood, allowing the organism to "wall " its infection off from the host's protective mechanisms rather effectively. There are 2 methods: slide test and tube test. The tube test is more accurate, but the slide test is faster.
- https://bio.libretexts.org/Learning_Objects/Laboratory_Experiments/Microbiology_Labs/Microbiology_Labs_I/06%3A_Oxygen_Requirements_and_AnaerobesAn excellent way to determine the oxygen needs of your bacterium is to grow it in different oxygen environments---atmospheric oxygen of 22%, no oxygen at all (GasPak jar), and reduced oxygen at less t...An excellent way to determine the oxygen needs of your bacterium is to grow it in different oxygen environments---atmospheric oxygen of 22%, no oxygen at all (GasPak jar), and reduced oxygen at less than 10% (candle jar)--and compare the quality and quantity of growth.
- https://bio.libretexts.org/Learning_Objects/Laboratory_Experiments/Microbiology_Labs/Microbiology_Labs_I/08%3A_Bacterial_Colony_MorphologyBacteria grow on solid media as colonies. A colony is defined as a visible mass of microorganisms all originating from a single mother cell, therefore a colony constitutes a clone of bacteria all gene...Bacteria grow on solid media as colonies. A colony is defined as a visible mass of microorganisms all originating from a single mother cell, therefore a colony constitutes a clone of bacteria all genetically alike.
- https://bio.libretexts.org/Learning_Objects/Laboratory_Experiments/Microbiology_Labs/Microbiology_Labs_I/12%3A_Oral_BiofilmsMost of the time in the microbiology lab, we study free-floating bacteria in broths or bacteria in colony forms, and generally in pure culture. However, in the real world bacteria are usually interact...Most of the time in the microbiology lab, we study free-floating bacteria in broths or bacteria in colony forms, and generally in pure culture. However, in the real world bacteria are usually interacting with other species in pretty sophisticated ecosystems. This assemblage of various organisms attached to a surface is called a biofilm , and the organisms that comprise it can include bacteria, plants, fungi, protozoa, and even multicellular animals, depending on where the biofilm is.
- https://bio.libretexts.org/Learning_Objects/Laboratory_Experiments/Microbiology_Labs/Microbiology_Labs_I/04%3A_Dilution_Worksheet_and_ProblemsColonies grow within the agar, as well as on top of the agar and below the agar (between the agar and the lower dish). To work the problem, you need 3 values---a colony count from the pour or spread p...Colonies grow within the agar, as well as on top of the agar and below the agar (between the agar and the lower dish). To work the problem, you need 3 values---a colony count from the pour or spread plates, a dilution factor for the dilution tube from which the countable agar plate comes, and the amount of the dilution that was plated on the agar plate. The exponent must show the number of decimal places that the decimal needs to be moved to change the number to standard notation.
- https://bio.libretexts.org/Learning_Objects/Laboratory_Experiments/Microbiology_Labs/Microbiology_Labs_I/27%3A_Oxidase_TestThe oxidase test is a key test to differentiate between the families of Pseudomonadaceae (ox +) and Enterobacteriaceae (ox -), and is useful for speciation and identification of many other bacteria, t...The oxidase test is a key test to differentiate between the families of Pseudomonadaceae (ox +) and Enterobacteriaceae (ox -), and is useful for speciation and identification of many other bacteria, those that have to use oxygen as the final electron acceptor in aerobic respiration. The enzyme cytochrome oxidase is involved with the reduction of oxygen at the end of the electron transport chain.
