17.7.5: Procedure for Filtration

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MEDIUM

2 plates of Trypticase Soy agar

ORGANISM

Trypticase Soy broth cultures of Micrococcus luteus

FILTRATION PROCEDURE (demonstration)

1. Using alcohol-flamed forceps, aseptically place a sterile membrane filter into a sterile filtration device.

2. Pour the culture of M. luteus into the top of the filter set-up.

3. Vacuum until all the liquid passes through the filter into the sterile flask.

4. With alcohol-flamed forceps, remove the filter and place it organism-side-up on the surface of a Trypticase Soy agar plate.

5. Using a sterile swab, streak the surface of another Trypticase Soy agar plate with the filtrate from the flask.

6. Incubate the plates at 37°C until the next lab period.

Contributors and Attributions

Dr. Gary Kaiser (COMMUNITY COLLEGE OF BALTIMORE COUNTY, CATONSVILLE CAMPUS)

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