17.7.5: Procedure for Filtration
- Page ID
- 122888
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MEDIUM
2 plates of Trypticase Soy agar
ORGANISM
Trypticase Soy broth cultures of Micrococcus luteus
FILTRATION PROCEDURE (demonstration)
1. Using alcohol-flamed forceps, aseptically place a sterile membrane filter into a sterile filtration device.
2. Pour the culture of M. luteus into the top of the filter set-up.
3. Vacuum until all the liquid passes through the filter into the sterile flask.
4. With alcohol-flamed forceps, remove the filter and place it organism-side-up on the surface of a Trypticase Soy agar plate.
5. Using a sterile swab, streak the surface of another Trypticase Soy agar plate with the filtrate from the flask.
6. Incubate the plates at 37°C until the next lab period.
Contributors and Attributions
Dr. Gary Kaiser (COMMUNITY COLLEGE OF BALTIMORE COUNTY, CATONSVILLE CAMPUS)