17.7.3: Procedure for Ultraviolet Radiation

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MEDIA

5 plates of Trypticase Soy agar

ORGANISM

Trypticase Soy broth culture of Serratia marcescens

ULTRAVIOLET RADIATION PROCEDURE (to be done by tables)

1. Using sterile swabs, streak all 5 Trypticase Soy agar plates with S. marcescens as follows:

a. Dip the swab into the culture.

b. Remove all of the excess liquid by pressing the swab against the side of the tube.

c. Streak the plate so as to cover the entire agar surface with organisms.

2. Expose 3 of the plates to UV light as follows:

a. Remove the lid of each plate and place a piece of cardboard with the letter "V" cut out of it over the top of the agar.

b. Expose the first plate to UV light for 1 second, the second plate for 3 seconds, and the third plate for 5 seconds.

c. Replace the lids and incubate the plates upside down at room temperature until the next lab period.

3. Leaving the lid on, lay the cardboard with the letter "V" cut out over the fourth plate and expose to UV light for 30 seconds. Incubate the plates upside down at room temperature with the other plates.

4. Use the fifth plate as a non-irradiated control and incubate the plates upside down at room temperature with the other plates.

Caution

Do not look directly at the UV light as it may harm the eyes.

Contributors and Attributions

Dr. Gary Kaiser (COMMUNITY COLLEGE OF BALTIMORE COUNTY, CATONSVILLE CAMPUS)