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Lab 5: Worksheet

  • Page ID
    23991
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    Name: ______________________________

    Section: _____________________________

    Student ID#:__________________________

    Work in groups on these problems. You should try to answer the questions without referring to your textbook. If you get stuck, try asking another group for help.

    1. Give the cardinal temperatures and descriptive group (page 31) for each bacterial species. (1.5)

    Escherichia coli :

    Geobacillus stearothermophilus :

    Pseudomonas fluorescens :

    2. Which bacterial species are acidophiles? (0.5)

    3. Which bacterial species are extreme halophiles? (1)

    4. Draw and label the growth on your plates. (1)

    5. A sample from a patient suspected of being infected with Clostridium difficile is left out too long before being taken to the lab for identification. Why would that be a problem? (1)

    TEMPERATURE:

    Escherichia coli
    10°C 20°C 30°C 40°C 50°C
    Table 1
    Table 2
    Table 3
    Table 4
    Geobacillus sterothermophilus
    10°C 20°C 30°C 40°C 50°C
    Table 1
    Table 2
    Table 3
    Table 4
    Pseudomonas fluorescens
    10°C 20°C 30°C 40°C 50°C
    Table 1
    Table 2
    Table 3
    Table 4

    pH:

    Lactobacillus plantarum
    pH 2 pH 4 pH 6 pH 8 pH 10
    Table 1
    Table 2
    Table 3
    Table 4
    Staphylococcus saprophyticus
    pH 2 pH 4 pH 6 pH 8 pH 10
    Table 1
    Table 2
    Table 3
    Table 4
    Alcaligenes faecalis
    pH 2 pH 4 pH 6 pH 8 pH 10
    Table 1
    Table 2
    Table 3
    Table 4

    SALINITY:

    Escherichia coli
    0% 2.5% 5% 10% 15% 20% 25%
    Table 1
    Table 2
    Table 3
    Table 4
    Halobacterium salinarum
    0% 2.5% 5% 10% 15% 20% 25%
    Table 1
    Table 2
    Table 3
    Table 4
    Vibrio alginolyticus
    0% 2.5% 5% 10% 15% 20% 25%
    Table 1
    Table 2
    Table 3
    Table 4
    Staphylococcus epidermis
    0% 2.5% 5% 10% 15% 20% 25%
    Table 1
    Table 2
    Table 3
    Table 4

    Lab 5: Worksheet is shared under a CC BY-SA license and was authored, remixed, and/or curated by Nazzy Pakpour & Sharon Horgan.

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