Lab 5: Worksheet

Last updated
Save as PDF

Page ID: 23991

\( \newcommand{\vecs}[1]{\overset { \scriptstyle \rightharpoonup} {\mathbf{#1}} } \) \( \newcommand{\vecd}[1]{\overset{-\!-\!\rightharpoonup}{\vphantom{a}\smash {#1}}} \)\(\newcommand{\id}{\mathrm{id}}\) \( \newcommand{\Span}{\mathrm{span}}\) \( \newcommand{\kernel}{\mathrm{null}\,}\) \( \newcommand{\range}{\mathrm{range}\,}\) \( \newcommand{\RealPart}{\mathrm{Re}}\) \( \newcommand{\ImaginaryPart}{\mathrm{Im}}\) \( \newcommand{\Argument}{\mathrm{Arg}}\) \( \newcommand{\norm}[1]{\| #1 \|}\) \( \newcommand{\inner}[2]{\langle #1, #2 \rangle}\) \( \newcommand{\Span}{\mathrm{span}}\) \(\newcommand{\id}{\mathrm{id}}\) \( \newcommand{\Span}{\mathrm{span}}\) \( \newcommand{\kernel}{\mathrm{null}\,}\) \( \newcommand{\range}{\mathrm{range}\,}\) \( \newcommand{\RealPart}{\mathrm{Re}}\) \( \newcommand{\ImaginaryPart}{\mathrm{Im}}\) \( \newcommand{\Argument}{\mathrm{Arg}}\) \( \newcommand{\norm}[1]{\| #1 \|}\) \( \newcommand{\inner}[2]{\langle #1, #2 \rangle}\) \( \newcommand{\Span}{\mathrm{span}}\)\(\newcommand{\AA}{\unicode[.8,0]{x212B}}\)

Name: ______________________________

Section: _____________________________

Student ID#:__________________________

Work in groups on these problems. You should try to answer the questions without referring to your textbook. If you get stuck, try asking another group for help.

1. Give the cardinal temperatures and descriptive group (page 31) for each bacterial species. (1.5)

Escherichia coli :

Geobacillus stearothermophilus :

Pseudomonas fluorescens :

2. Which bacterial species are acidophiles? (0.5)

3. Which bacterial species are extreme halophiles? (1)

4. Draw and label the growth on your plates. (1)

5. A sample from a patient suspected of being infected with Clostridium difficile is left out too long before being taken to the lab for identification. Why would that be a problem? (1)

TEMPERATURE:

	Escherichia coli
	10°C	20°C	30°C	40°C	50°C
Table 1
Table 2
Table 3
Table 4

	Geobacillus sterothermophilus
	10°C	20°C	30°C	40°C	50°C
Table 1
Table 2
Table 3
Table 4

	Pseudomonas fluorescens
	10°C	20°C	30°C	40°C	50°C
Table 1
Table 2
Table 3
Table 4

pH:

	Lactobacillus plantarum
	pH 2	pH 4	pH 6	pH 8	pH 10
Table 1
Table 2
Table 3
Table 4

	Staphylococcus saprophyticus
	pH 2	pH 4	pH 6	pH 8	pH 10
Table 1
Table 2
Table 3
Table 4

	Alcaligenes faecalis
	pH 2	pH 4	pH 6	pH 8	pH 10
Table 1
Table 2
Table 3
Table 4

SALINITY:

	Escherichia coli
	0%	2.5%	5%	10%	15%	20%	25%
Table 1
Table 2
Table 3
Table 4

	Halobacterium salinarum
	0%	2.5%	5%	10%	15%	20%	25%
Table 1
Table 2
Table 3
Table 4

	Vibrio alginolyticus
	0%	2.5%	5%	10%	15%	20%	25%
Table 1
Table 2
Table 3
Table 4

	Staphylococcus epidermis
	0%	2.5%	5%	10%	15%	20%	25%
Table 1
Table 2
Table 3
Table 4