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6: Mammalian Cell Culture Fundamentals

  • Page ID
    154031
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    • 6.1: Principles of Mammalian Cell Culture
      This page describes the basic principles of mammalian cell culture, including the types of cells, cultures, and culture conditions used today. The concept of biosafety and the four biosafety levels used in culture facilities are also described.
    • 6.2: Principles of Aseptic Technique
      This page describes the principles of aseptic technique that is used to maintain the sterility of mammalian cell cultures.  Specific aspects of aseptic technique, such as autoclaving and filtration is given, along with a description of some of the more common micro-organisms that can contaminate a cell culture.
    • 6.3: Cell Culture Equipment
      This page outlines some of the basic equipment used in a mammalian cell culture facility, including the biosafety cabinet, the incubator, and the hemacytometer.


    6: Mammalian Cell Culture Fundamentals is shared under a CC BY 4.0 license and was authored, remixed, and/or curated by LibreTexts.

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