3.37: Human Microbiome

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Page ID: 91013

Rosanna Hartline
Lemoore College

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Preparation

2 TSA plates per student

Materials

2 TSA plates per student
sterile cotton swabs (2 per student)
DI or distilled water
markers
materials for Gram staining
- plain microscope slides (at least 1 per group)
- Bunsen burners (1 per group)
- inoculating loops (1 per group)
- droppers with saline, DI water, or distilled water
- slide warmer
- wood test tube clamps/holders (1 per group)
- small chemical waste containers (1 per group)
- crystal violet in a dropper bottle (1 per group or enough for two groups to share one bottle)
- Gram's iodine in a dropper bottle (1 per group or enough for two groups to share one bottle)
- 95% ethanol in a dropper bottle (1 per group or enough for two groups to share one bottle)
- safranin in a dropper bottle (1 per group or enough for two groups to share one bottle)
- distilled or DI water in squirt bottles (1 per group)
- bibulous paper (enough for each groups to blot their slides)
- light microscopes (1 per group)
- (optional, but recommended) Wax pencils (1 per group)
- (if using oil immersion) immersion oil dropper bottles
- (if using oil immersion) lens paper

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