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3.37: Human Microbiome

  • Page ID
    91013
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    Preparation

    • 2 TSA plates per student

    Materials

    • 2 TSA plates per student
    • sterile cotton swabs (2 per student)
    • DI or distilled water
    • markers
    • materials for Gram staining
      • plain microscope slides (at least 1 per group)
      • Bunsen burners (1 per group)
      • inoculating loops (1 per group)
      • droppers with saline, DI water, or distilled water
      • slide warmer
      • wood test tube clamps/holders (1 per group)
      • small chemical waste containers (1 per group)
      • crystal violet in a dropper bottle (1 per group or enough for two groups to share one bottle)
      • Gram's iodine in a dropper bottle (1 per group or enough for two groups to share one bottle)
      • 95% ethanol in a dropper bottle (1 per group or enough for two groups to share one bottle)
      • safranin in a dropper bottle (1 per group or enough for two groups to share one bottle)
      • distilled or DI water in squirt bottles (1 per group)
      • bibulous paper (enough for each groups to blot their slides)
      • light microscopes (1 per group)
      • (optional, but recommended) Wax pencils (1 per group)
      • (if using oil immersion) immersion oil dropper bottles
      • (if using oil immersion) lens paper

    This page titled 3.37: Human Microbiome is shared under a CC BY-NC-SA 4.0 license and was authored, remixed, and/or curated by Rosanna Hartline.

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