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3.36: Bacterial Susceptibility to Antibiotics (Kirby-Bauer Test)

  • Page ID
    91012
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    Preparation

    • TSA petri plates (enough for 3 per group)
    • 18-24 hour TSB cultures of Staphylococcus aureus (one per group or enough for groups to share)

    Materials

    • TSA petri plates (enough for 3 per group)
    • 18-24 hour TSB cultures of Staphylococcus aureus (one per group or enough for groups to share)
    • test tube racks (1 per group)
    • Bunsen burners (1 per group)
    • strikers (1 per group)
    • sterile cotton swabs (3 per group)
    • waste containers with disinfectant for used cotton swabs (1 per group)
    • tweezers (1 per antibiotic type - label tweezers so they do not get mixed up)
    • antibiotic disks (one of each type per group):
      • erythromycin (15 µg)
      • penicillin G (10 units)
      • streptomycin (10 µg)
      • tetracycline (30 µg)

    This page titled 3.36: Bacterial Susceptibility to Antibiotics (Kirby-Bauer Test) is shared under a CC BY-NC-SA 4.0 license and was authored, remixed, and/or curated by Rosanna Hartline.

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