17.1H: Production of Vaccines, Antibiotics, and Hormones
- Page ID
- 13344
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Production of Vaccines, Antibiotics, and Hormones
Vaccines
Traditional vaccination strategies use weakened or inactive forms of microorganisms to mount the initial immune response. Modern techniques use the genes of microorganisms cloned into vectors to mass produce the desired antigen. The antigen is then introduced into the body to stimulate the primary immune response and trigger immune memory. Genes cloned from the influenza virus have been used to combat the constantly-changing strains of this virus.
Antibiotics
Antibiotics are biotechnological products that inhibit bacterial growth or kill bacteria. They are naturally produced by microorganisms, such as fungi, to attain an advantage over bacterial populations. Antibiotics are produced on a large scale by cultivating and manipulating fungal cells. Many antibacterial compounds are classified on the basis of their chemical or biosynthetic origin into natural, semisynthetic, and synthetic. Another classification system is based on biological activity. In this classification, antibiotics are divided into two broad groups according to their biological effect on microorganisms: bactericidal agents kill bacteria, and bacteriostatic agents slow down or stall bacterial growth.
Hormones
Recombinant DNA technology was used to produce large-scale quantities of human insulin (a hormone) in E. coli as early as 1978. Previously, it was only possible to treat diabetes with pig insulin, which caused allergic reactions in humans because of differences in the gene product. In recent times, human growth hormone (HGH) has been used to treat growth disorders in children. The HGH gene was cloned from a cDNA library and inserted into E. coli cells by cloning it into a bacterial vector. The bacteria was then grown and the hormone isolated, enabling large scale commercial production.
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Key Points
- Vaccines use weakened or inactive forms of microorganisms to mount the initial immune response through the use of antigens, which are produced through use the genes of microbes that are cloned into vectors.
- Antibiotics, agents that inhibit bacterial growth or kill bacteria, are produced by cultivating and manipulating fungal cells.
- Hormones, such as the human growth hormone (HGH), can be formulated through recombinant DNA technology; for example, HGH can be cloned from a cDNA library and inserted into E. coli cells by cloning it into a bacterial vector.
Key Terms
- bactericidal: that which kills bacteria
- bacteriostatic: that which slows down or stalls bacterial growth
- antigen: a substance that binds to a specific antibody; may cause an immune response