- Explain the formation of the action potential in neurons
A neuron can receive input from other neurons via a chemical called a neurotransmitter. If this input is strong enough, the neuron will pass the signal to downstream neurons. Transmission of a signal within a neuron (in one direction only, from dendrite to axon terminal) is carried out by the opening and closing of voltage-gated ion channels, which cause a brief reversal of the resting membrane potential to create an action potential. As an action potential travels down the axon, the polarity changes across the membrane. Once the signal reaches the axon terminal, it stimulates other neurons.
Depolarization and the Action Potential
When neurotransmitter molecules bind to receptors located on a neuron’s dendrites, voltage-gated ion channels open. At excitatory synapses, positive ions flood the interior of the neuron and depolarize the membrane, decreasing the difference in voltage between the inside and outside of the neuron. A stimulus from a sensory cell or another neuron depolarizes the target neuron to its threshold potential (-55 mV), and Na+ channels in the axon hillock open, starting an action potential. Once the sodium channels open, the neuron completely depolarizes to a membrane potential of about +40 mV. The action potential travels down the neuron as Na+ channels open.
Hyperpolarization and Return to Resting Potential
Action potentials are considered an “all-or nothing” event. Once the threshold potential is reached, the neuron completely depolarizes. As soon as depolarization is complete, the cell “resets” its membrane voltage back to the resting potential. The Na+ channels close, beginning the neuron’s refractory period. At the same time, voltage-gated K+ channels open, allowing K+ to leave the cell. As K+ ions leave the cell, the membrane potential once again becomes negative. The diffusion of K+ out of the cell hyperpolarizes the cell, making the membrane potential more negative than the cell’s normal resting potential. At this point, the sodium channels return to their resting state, ready to open again if the membrane potential again exceeds the threshold potential. Eventually, the extra K+ ions diffuse out of the cell through the potassium leakage channels, bringing the cell from its hyperpolarized state back to its resting membrane potential.
Myelin and Propagation of the Action Potential
For an action potential to communicate information to another neuron, it must travel along the axon and reach the axon terminals where it can initiate neurotransmitter release. The speed of conduction of an action potential along an axon is influenced by both the diameter of the axon and the axon’s resistance to current leak. Myelin acts as an insulator that prevents current from leaving the axon, increasing the speed of action potential conduction. Diseases like multiple sclerosis cause degeneration of the myelin, which slows action potential conduction because axon areas are no longer insulated so the current leaks.
A node of Ranvier is a natural gap in the myelin sheath along the axon. These unmyelinated spaces are about one micrometer long and contain voltage gated Na+ and K+ channels. The flow of ions through these channels, particularly the Na+ channels, regenerates the action potential over and over again along the axon. Action potential “jumps” from one node to the next in saltatory conduction. If nodes of Ranvier were not present along an axon, the action potential would propagate very slowly; Na+ and K+ channels would have to continuously regenerate action potentials at every point along the axon. Nodes of Ranvier also save energy for the neuron since the channels only need to be present at the nodes and not along the entire axon.
- Action potentials are formed when a stimulus causes the cell membrane to depolarize past the threshold of excitation, causing all sodium ion channels to open.
- When the potassium ion channels are opened and sodium ion channels are closed, the cell membrane becomes hyperpolarized as potassium ions leave the cell; the cell cannot fire during this refractory period.
- The action potential travels down the axon as the membrane of the axon depolarizes and repolarizes.
- Myelin insulates the axon to prevent leakage of the current as it travels down the axon.
- Nodes of Ranvier are gaps in the myelin along the axons; they contain sodium and potassium ion channels, allowing the action potential to travel quickly down the axon by jumping from one node to the next.
- action potential: a short term change in the electrical potential that travels along a cell
- depolarization: a decrease in the difference in voltage between the inside and outside of the neuron
- hyperpolarize: to increase the polarity of something, especially the polarity across a biological membrane
- node of Ranvier: a small constriction in the myelin sheath of axons
- saltatory conduction: the process of regenerating the action potential at each node of Ranvier