2.4: Appendix IV- Using the Qubit
- Page ID
- 67603
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How to Use the Qubit Fluorometer
Watch the YouTube video regarding questions for the Qubit Fluorometer.
(https://www.youtube.com/watch?v=z5g6pAFAWEc)
The Qubit allows you to measure the concentration of DNA, RNA, and protein in a couple of microliters of the solution. It is best to use 1.5\(\mu L\) to 2\(\mu L\) for accurate measurements. Some models have the ability to measure fluorescently labeled molecules.
Procedure:
- Calibration:
- On the “Home Screen,” choose the type of assay for which you want to run the standards. Standards Screen is automatically displayed.
- The screen will prompt you to choose between reading new standards and using the previous calibration. Press “Yes” to read new standards.
- Insert Standard #2, and press “Read.” Ensure that you are using the Standard #2 appropriate for the assay you are performing.
- Calibration is complete. The new standards graph with data points for standards connected by a line appears on the screen.
- Reading Samples:
- Choose “Sample” to go on the Sample Screen
- Insert a sample into the Sample Chamber and press “Read.” The measurement takes approximately 3 seconds. Upon the completion of the measurement, the result is displayed on the screen. The number displayed is the concentration of the nucleic acid in the assay tube.
- To read the next sample, remove the sample from the Sample Chamber, insert the next sample, and press “Read Next Sample.”
- Repeat until all samples are read.