8.7: Cheek Cell DNA Extraction
- Page ID
- 24777
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Using Cytobrush
- Use a sterile cytobrush and insert into mouth.
- Brush the cytobrush on the inside of your cheek 25 times.
- Swirl the cytobrush in 100 μl of Chelex suspension (10% w/v).
- Place a centrifuge tube with Chelex and cell suspension on a 100 °C heat block for 10 minutes.
- Centrifuge the tubes at maximum speed for 5 minutes.
- DNA is in the supernatant. Avoid beads at the bottom.
- Store DNA at -20 °C.
PCR with PCR Beads
- Add 22 μl of the primer mix (forward and reverse) to beads.
- Ensure that the bead is dissolved.
- Add 3 μl of DNA.