8.7: Cheek Cell DNA Extraction

Using Cytobrush

1. Use a sterile cytobrush and insert into mouth.
2. Brush the cytobrush on the inside of your cheek 25 times.
3. Swirl the cytobrush in 100 μl of Chelex suspension (10% w/v).
4. Place a centrifuge tube with Chelex and cell suspension on a 100 °C heat block for 10 minutes.
5. Centrifuge the tubes at maximum speed for 5 minutes.
6. DNA is in the supernatant. Avoid beads at the bottom.
7. Store DNA at -20 °C.

PCR with PCR Beads

1. Add 22 μl of the primer mix (forward and reverse) to beads.
2. Ensure that the bead is dissolved.
3. Add 3 μl of DNA.