21.1: The Biodiversity Crisis

Learning Objectives

By the end of this section, you will be able to do the following:

• Define biodiversity in terms of species diversity and abundance
• Describe biodiversity as the equilibrium of naturally fluctuating rates of extinction and speciation
• Identify historical causes of high extinction rates in Earth’s history

Traditionally, ecologists have measured biodiversity, a general term for the number of species present in the biosphere, by taking into account both the number of species and their relative abundance to each other. Biodiversity can be estimated at a number of levels of organization of living organisms. These estimation indices, which came from information theory, are most useful as a first step in quantifying biodiversity between and within ecosystems; they are less useful when the main concern among conservation biologists is simply the loss of biodiversity. However, biologists recognize that measures of biodiversity, in terms of species diversity, may help focus efforts to preserve the biologically or technologically important elements of biodiversity.

The Lake Victoria cichlids provide an example with which we can begin to understand biodiversity. The biologists studying cichlids in the 1980s discovered hundreds of cichlid species representing a variety of specializations to specialized habitat types and specific feeding strategies: such as eating plankton floating in the water, scraping/eating algae from rocks, eating insect larvae from the lake bottom, and eating the eggs of other species of cichlid. The cichlids of Lake Victoria are the product of a complex adaptive radiation. An adaptive radiation is a rapid (less than three million years in the case of the Lake Victoria cichlids) branching through speciation of a phylogenetic clade into many closely related species. Typically, the species “radiate” into different habitats and niches. The Galápagos Island finches are an example of a modest adaptive radiation with 15 species. The cichlids of Lake Victoria are an example of a spectacular adaptive radiation that formerly included about 500 species.

At the time biologists were making this discovery, some species began to quickly disappear. A culprit in these declines was the Nile perch, a species of large predatory fish that was introduced to Lake Victoria by fisheries to feed the people living around the lake. The Nile perch was introduced in 1963, but its populations did not begin to surge until the 1980s. The perch population grew by consuming cichlids, driving species after species to the point of extinction (the disappearance of a species). In fact, there were several factors that played a role in the extinction of perhaps 200 cichlid species in Lake Victoria: the Nile perch, declining lake water quality due to agriculture and land clearing on the shores of Lake Victoria, and increased fishing pressure. Scientists had not even catalogued all of the species present—so many were lost that were never named. The diversity is now a shadow of what it once was.

The cichlids of Lake Victoria are a thumbnail sketch of contemporary rapid species loss that occurs all over Earth that is caused primarily by human activity. Extinction is a natural process of macroevolution that occurs at the rate of about one out of 1 million species becoming extinct per year. The fossil record reveals that there have been five periods of mass extinction in history with much higher rates of species loss, and the rate of species loss today is comparable to those periods of mass extinction. However, there is a major difference between the previous mass extinctions and the current extinction we are experiencing: human activity. Specifically, three human activities have a major impact: 1) destruction of habitat, 2) introduction of exotic species, and 3) over-harvesting. Predictions of species loss within the next century, a tiny amount of time on geological timescales, range from 10 percent to 50 percent. Extinctions on this scale have only happened five other times in the history of the planet, and these extinctions were caused by cataclysmic events that changed the course of the history of life in each instance.

Types of Biodiversity

Scientists generally accept that the term biodiversity describes the number and kinds of species and their abundance in a given location or on the planet. Species can be difficult to define, but most biologists still feel comfortable with the concept and are able to identify and count eukaryotic species in most contexts. Biologists have also identified alternate measures of biodiversity, some of which are important for planning how to preserve biodiversity.

Genetic diversity is one of those alternate concepts. Genetic diversity, or genetic variation defines the raw material for evolution and adaptation in a species. A species’ future potential for adaptation depends on the genetic diversity held in the genomes of the individuals in populations that make up the species. The same is true for higher taxonomic categories. A genus with very different types of species will have more genetic diversity than a genus with species that are genetically similar and have similar ecologies. If there were a choice between one of these genera of species being preserved, the one with the greatest potential for subsequent evolution is the most genetically diverse one.

Many genes code for proteins, which in turn carry out the metabolic processes that keep organisms alive and reproducing. Genetic diversity can be measured as chemical diversity in that different species produce a variety of chemicals in their cells, both the proteins as well as the products and byproducts of metabolism. This chemical diversity has potential benefit for humans as a source of pharmaceuticals, so it provides one way to measure diversity that is important to human health and welfare.

Humans have generated diversity in domestic animals, plants, and fungi, among many other organisms. This diversity is also suffering losses because of migration, market forces, and increasing globalism in agriculture, especially in densely populated regions such as China, India, and Japan. The human population directly depends on this diversity as a stable food source, and its decline is troubling biologists and agricultural scientists.

It is also useful to define ecosystem diversity, meaning the number of different ecosystems on the planet or within a given geographic area (Figure 47.2). Whole ecosystems can disappear even if some of the species might survive by adapting to other ecosystems. The loss of an ecosystem means the loss of interactions between species, the loss of unique features of coadaptation, and the loss of biological productivity that an ecosystem is able to create. An example of a largely extinct ecosystem in North America is the prairie ecosystem. Prairies once spanned central North America from the boreal forest in northern Canada down into Mexico. They are now all but gone, replaced by crop fields, pasture lands, and suburban sprawl. Many of the species survive elsewhere, but the hugely productive ecosystem that was responsible for creating the most productive agricultural soils in the United States is now gone. As a consequence, native soils are disappearing or must be maintained and enhanced at great expense.

Figure 47.2 The variety of ecosystems on Earth—from (a) coral reef to (b) prairie—enables a great diversity of species to exist. (credit a: modification of work by Jim Maragos, USFWS; credit b: modification of work by Jim Minnerath, USFWS)

Current Species Diversity

Despite considerable effort, knowledge of the species that inhabit the planet is limited and always will be because of a continuing lack of financial resources and political willpower. A recent estimate suggests that the eukaryote species for which science has names, about 1.5 million species, account for less than 20 percent of the total number of eukaryote species present on the planet (8.7 million species, by one estimate). Estimates of numbers of prokaryotic species are largely guesses, but biologists agree that science has only begun to catalog their diversity. Even with what is known, there is no central repository of names or samples of the described species; therefore, there is no way to be sure that the 1.5 million descriptions is an accurate accounting. It is a best guess based on the opinions of experts in different taxonomic groups. Given that Earth is losing species at an accelerating pace, science is very much in the place it was with the Lake Victoria cichlids: knowing little about what is being lost. Table 47.1 presents recent estimates of biodiversity in different groups.

Estimates of the Numbers of Described and Predicted Species by Taxonomic Group

	Mora et al. 2011^1		Chapman 2009^2		Groombridge & Jenkins 2002^3
	Described	Predicted	Described	Predicted	Described	Predicted
Animalia	1,124,516	9,920,000	1,424,153	6,836,330	1,225,500	10,820,000
Chromista	17,892	34,900	25,044	200,500	—	—
Fungi	44,368	616,320	98,998	1,500,000	72,000	1,500,000
Plantae	224,244	314,600	310,129	390,800	270,000	320,000
Protozoa	16,236	72,800	28,871	1,000,000	80,000	600,000
Prokaryotes	—	—	10,307	1,000,000	10,175	—
Total	1,438,769	10,960,000	1,897,502	10,897,630	1,657,675	13,240,000

Table 47.1

There are various initiatives to catalog described species in accessible ways, and the internet is facilitating that effort. Nevertheless, it has been pointed out that at the current rate of new species descriptions (which according to the State of Observed Species Report is 17,000 to 20,000 new species per year), it will take close to 500 years to finish describing life on this planet.⁴ Over time, the task becomes both increasingly difficult and increasingly easier as extinction removes species from the planet.

Naming and counting species may seem like an unimportant pursuit given the other needs of humanity, but determining biodiversity it is not simply an accounting of species. Describing a species is a complex process through which biologists determine an organism’s unique characteristics and whether or not that organism belongs to any other described species or genus. It allows biologists to find and recognize the species after the initial discovery, and allows them to follow up on questions about its biology. In addition, the unique characteristics of each species make it potentially valuable to humans or other species on which humans depend.

Patterns of Biodiversity

Biodiversity is not evenly distributed on Earth. Lake Victoria contained almost 500 species of cichlids alone, ignoring the other fish families present in the lake. All of these species were found only in Lake Victoria; therefore, the 500 species of cichlids were endemic. Endemic species are found in only one location. Endemics with highly restricted distributions are particularly vulnerable to extinction. Higher taxonomic levels, such as genera and families, can also be endemic. Lake Michigan contains about 79 species of fish, many of which are found in other lakes in North America. What accounts for the difference in fish diversity in these two lakes? Lake Victoria is an ancient tropical lake, while Lake Michigan is a recently formed temperate lake. Lake Michigan in its present form is only about 7,000 years old, while Lake Victoria in its present form is about 15,000 years old, although its basin is about 400,000 years in age. Biogeographers have suggested these two factors, latitude and age, are two of several hypotheses to explain biodiversity patterns on the planet.

Career Connection

Career Connection

BiogeographerBiogeography is the study of the distribution of the world’s species—both in the past and in the present. The work of biogeographers is critical to understanding our physical environment, how the environment affects species, and how environmental changes impact the distribution of a species; it has also been critical to developing modern evolutionary theory. Biogeographers need to understand both biology and ecology. They also need to be well-versed in evolutionary studies, soil science, and climatology.

There are three main fields of study under the heading of biogeography: ecological biogeography, historical biogeography (called paleobiogeography), and conservation biogeography. Ecological biogeography studies the current factors affecting the distribution of plants and animals. Historical biogeography, as the name implies, studies the past distribution of species. Conservation biogeography, on the other hand, is focused on the protection and restoration of species based upon known historical and current ecological information. Each of these fields considers both zoogeography and phytogeography—the past and present distribution of animals and plants.

One of the oldest observed patterns in ecology is that species biodiversity in almost every taxonomic group increases as latitude declines. In other words, biodiversity increases closer to the equator (Figure 47.3).

Figure 47.3 This map illustrates the number of amphibian species across the globe and shows the trend toward higher biodiversity at lower latitudes. A similar pattern is observed for most taxonomic groups. The white areas indicate a lack of data in this particular study.

It is not yet clear why biodiversity increases closer to the equator, but scientists have several hypotheses. One factor may be the greater age of the ecosystems in the tropics versus those in temperate regions; the temperate regions were largely devoid of life or were drastically reduced during the last glaciation. The idea is that greater age provides more time for speciation. Another possible explanation is the increased direct energy the tropics receive from the sun versus the decreased intensity of the solar energy that temperate and polar regions receive. Tropical ecosystem complexity may promote speciation by increasing the heterogeneity, or number of ecological niches, in the tropics relative to higher latitudes. The greater heterogeneity provides more opportunities for coevolution, specialization, and perhaps greater selection pressures leading to population differentiation. However, this hypothesis suffers from some circularity—ecosystems with more species encourage speciation, but how did they get more species to begin with?

The tropics have been perceived as being more stable than temperate regions, which have a pronounced climate and day-length seasonality. The tropics have their own forms of seasonality, such as rainfall, but they are generally assumed to be more stable environments and this stability might promote speciation into highly specialized niches.

Regardless of the mechanisms, it is certainly true that all levels of biodiversity are greatest in the tropics. Additionally, the rate of endemism is highest, and there are more biodiversity “hotspots.” However, this richness of diversity also means that knowledge of species is unfortunately very low, and there is a high potential for biodiversity loss.

Conservation of Biodiversity

In 1988, British environmentalist Norman Myers developed a conservation concept to identify areas rich in species and at significant risk for species loss: biodiversity hotspots. Biodiversity hotspots are geographical areas that contain high numbers of endemic species. The purpose of the concept was to identify important locations on the planet for conservation efforts, a kind of conservation triage. By protecting hotspots, governments are able to protect a larger number of species. The original criteria for a hotspot included the presence of 1500 or more endemic plant species and 70 percent of the area disturbed by human activity. There are now 34 biodiversity hotspots (Figure 47.4) containing large numbers of endemic species, which include half of Earth’s endemic plants.

Figure 47.4 Conservation International has identified 34 biodiversity hotspots, which cover only 2.3 percent of the Earth’s surface but have endemic to them 42 percent of the terrestrial vertebrate species and 50 percent of the world’s plants.

Biodiversity Change through Geological Time

The number of species on the planet, or in any geographical area, is the result of an equilibrium of two evolutionary processes that are continuously ongoing: speciation and extinction. Both are natural “birth” and “death” processes of macroevolution. When speciation rates begin to outstrip extinction rates, the number of species will increase; likewise, the number of species will decrease when extinction rates begin to overtake speciation rates. Throughout Earth’s history, these two processes have fluctuated—sometimes leading to dramatic changes in the number of species on Earth as reflected in the fossil record (Figure 47.5).

Figure 47.5 Percent extinction occurrences as reflected in the fossil record have fluctuated throughout Earth’s history. Sudden and dramatic losses of biodiversity, called mass extinctions, have occurred five times.

Paleontologists have identified five strata in the fossil record that appear to show sudden and dramatic (greater than half of all extant species disappearing from the fossil record) losses in biodiversity. These are called mass extinctions. There are many lesser, yet still dramatic, extinction events, but the five mass extinctions have attracted the most research. An argument can be made that the five mass extinctions are only the five most extreme events in a continuous series of large extinction events throughout the Phanerozoic (since 542 million years ago). In most cases, the hypothesized causes are still controversial; however, the most recent mass extinction event seems clear.

The Five Mass Extinctions

The fossil record of the mass extinctions was the basis for defining periods of geological history, so they typically occur at the transition point between geological periods. The transition in fossils from one period to another reflects the dramatic loss of species and the gradual origin of new species. These transitions can be seen in the rock strata. Table 47.2 provides data on the five mass extinctions.

Mass Extinctions

Geological Period	Mass Extinction Name	Time (millions of years ago)
Ordovician–Silurian	end-Ordovician O–S	450–440
Late Devonian	end-Devonian	375–360
Permian–Triassic	end-Permian	251
Triassic–Jurassic	end-Triassic	205
Cretaceous–Paleogene	end-Cretaceous K–Pg (K–T)	65.5

Table 47.2 This table shows the names and dates for the five mass extinctions in Earth’s history.

The Ordovician-Silurian extinction event is the first recorded mass extinction and the second largest. During this period, about 85 percent of marine species (few species lived outside the oceans) became extinct. The main hypothesis for its cause is a period of glaciation and then warming. The extinction event actually consists of two extinction events separated by about 1 million years. The first event was caused by cooling, and the second event was due to the subsequent warming. The climate changes affected temperatures and sea levels. Some researchers have suggested that a gamma-ray burst, caused by a nearby supernova, was a possible cause of the Ordovician-Silurian extinction. The gamma-ray burst would have stripped away the Earth’s protective ozone layer, allowing intense ultraviolet radiation from the sun to reach the surface of the earth—and may account for climate changes observed at the time. The hypothesis is very speculative, and extraterrestrial influences on Earth’s history are an active line of research. Recovery of biodiversity after the mass extinction took from 5 to 20 million years, depending on the location.

The late Devonian extinction may have occurred over a relatively long period of time. It appears to have mostly affected marine species and not so much the plants or animals inhabiting terrestrial habitats. The causes of this extinction are poorly understood.

The end-Permian extinction was the largest in the history of life. Indeed, an argument could be made that Earth became nearly devoid of life during this extinction event. Estimates are that 96 percent of all marine species and 70 percent of all terrestrial species were lost. It was at this time, for example, that the trilobites, a group that survived the Ordovician–Silurian extinction, became extinct. The causes for this mass extinction are not clear, but the leading suspect is extended and widespread volcanic activity that led to a runaway global-warming event. The oceans became largely anoxic, suffocating marine life. Terrestrial tetrapod diversity took 30 million years to recover after the end-Permian extinction. The Permian extinction dramatically altered Earth’s biodiversity makeup and the course of evolution.

The causes of the Triassic–Jurassic extinction event are not clear, and researchers argue hypotheses including climate change, asteroid impact, and volcanic eruptions. The extinction event occurred just before the breakup of the supercontinent Pangaea, although recent scholarship suggests that the extinctions may have occurred more gradually throughout the Triassic.

The causes of the end-Cretaceous extinction event are the ones that are best understood. It was during this extinction event about 65 million years ago that the majority of the dinosaurs, the dominant vertebrate group for millions of years, disappeared from the planet (with the exception of a theropod clade that gave rise to birds).

The cause of this extinction is now understood to be the result of a cataclysmic impact of a large meteorite, or asteroid, off the coast of what is now the Yucatán Peninsula. This hypothesis, proposed first in 1980, was a radical explanation based on a sharp spike in the levels of iridium (which enters our atmosphere from meteors at a fairly constant rate but is otherwise absent on Earth’s surface) in the rock stratum that marks the boundary between the Cretaceous and Paleogene periods (Figure 47.6). This boundary marked the disappearance of the dinosaurs in fossils as well as many other taxa. The researchers who discovered the iridium spike interpreted it as a rapid influx of iridium from space to the atmosphere (in the form of a large asteroid) rather than a slowing in the deposition of sediments during that period. It was a radical explanation, but the report of an appropriately aged and sized impact crater in 1991 made the hypothesis more believable. Now an abundance of geological evidence supports the theory. Recovery times for biodiversity after the end-Cretaceous extinction are shorter, in geological time, than for the end-Permian extinction, on the order of 10 million years.

Another possibility, perhaps coincidental with the impact of the Yucatan asteroid, was extensive volcanism that began forming about 66 million years ago, about the same time as the Yucatan asteroid impact, at the end of the Cretaceous. The lava flows covered over 50 percent of what is now India. The release of volcanic gases, particularly sulfur dioxide, during the formation of the traps contributed to climate change, which may have induced the mass extinction.

Visual Connection

Visual Connection

Figure 47.6 In 1980, Luis and Walter Alvarez, Frank Asaro, and Helen Michels discovered, across the world, a spike in the concentration of iridium within the sedimentary layer at the K–Pg boundary. These researchers hypothesized that this iridium spike was caused by an asteroid impact that resulted in the K–Pg mass extinction. In the photo, the iridium layer is the light band. (credit: USGS)

Scientists measured the relative abundance of fern spores above and below the K–Pg boundary in this rock sample. Which of the following statements most likely represents their findings?

1. An abundance of fern spores from several species was found below the K–Pg boundary, but none was found above.
2. An abundance of fern spores from several species was found above the K–Pg boundary, but none was found below.
3. An abundance of fern spores was found both above and below the K–Pg boundary, but only one species was found below the boundary, and many species were found above the boundary.
4. Many species of fern spores were found both above and below the boundary, but the total number of spores was greater below the boundary.

Link to Learning

Link to Learning

Explore this interactive website about mass extinctions.

The Pleistocene Extinction

The Pleistocene Extinction is one of the lesser extinctions, and a recent one. It is well known that the North American, and to some degree Eurasian, megafauna—large vertebrate animals—disappeared toward the end of the last glaciation period. The extinction appears to have happened in a relatively restricted time period of 10,000–12,000 years ago. In North America, the losses were quite dramatic and included the woolly mammoths (with an extant population existing until about 4,000 years ago in isolation on Wrangel Island, Canada), mastodon, giant beavers, giant ground sloths, saber-toothed cats, and the North American camel, just to name a few. In the early 1900s, scientists first suggested the possibility that over-hunting caused the rapid extinction of these large animals. Research into this hypothesis continues today.

In general, the timing of the Pleistocene extinctions correlated with the arrival of paleo-humans, perhaps as long as 40,000 years ago, and not with climate-change events, which is the main competing hypothesis for these extinctions. The extinctions began in Australia about 40,000 to 50,000 years ago, just after the arrival of humans in the area: a marsupial lion, a giant one-ton wombat, and several giant kangaroo species disappeared. In North America, the extinctions of almost all of the large mammals occurred 10,000–12,000 years ago. All that are left are the smaller mammals such as bears, elk, moose, and cougars. Finally, on many remote oceanic islands, the extinctions of many species occurred coincidentally with human arrivals. Not all of the islands had large animals, but when there were large animals, they were often forced into extinction. Madagascar was colonized about 2,000 years ago and the large mammals that lived there became extinct. Eurasia and Africa do not show this pattern, but they also did not experience a recent arrival of hunter-gatherer humans. Rather, humans arrived in Eurasia hundreds of thousands of years ago. This topic remains an area of active research and hypothesizing. It seems clear that even if climate played a role, in most cases human hunting precipitated the extinctions.

Recent Extinctions

The sixth, or Holocene, mass extinction appears to have begun earlier than previously believed and is largely due to the disruptive activities of modern Homo sapiens. Since the beginning of the Holocene period, there are numerous recent extinctions of individual species that are recorded in human writings. Most of these are coincident with the expansion of the European colonies since the 1500s.

One of the earlier and popularly known examples is the dodo bird. The odd pigeon-like bird lived in the forests of Mauritius (an island in the Indian Ocean) and became extinct around 1662. The dodo was hunted for its meat by sailors and was easy prey because it approached people without fear (the dodo had not evolved with humans). Pigs, rats, and dogs brought to the island by European ships also killed dodo young and eggs.

Steller's sea cow became extinct in 1768; it was related to the manatee and probably once lived along the northwest coast of North America. Steller's sea cow was first discovered by Europeans in 1741 and was overhunted for meat and oil. The last sea cow was killed in 1768. That amounts to just 27 years between the sea cow’s first contact with Europeans and extinction of the species!

Since 1900, a variety of species have gone extinct, including the following:

• In 1914, the last living passenger pigeon died in a zoo in Cincinnati, Ohio. This species had once darkened the skies of North America during its migrations, but it was overhunted and suffered from habitat loss that resulted from the clearing of forests for farmland.
• The Carolina parakeet, once common in the eastern United States, died out in 1918. It suffered habitat loss and was hunted to prevent it from eating orchard fruit. (The parakeet ate orchard fruit because its native foods were destroyed to make way for farmland.)
• The Japanese sea lion, which inhabited a broad area around Japan and the coast of Korea, became extinct in the 1950s due to fishermen.
• The Caribbean monk seal was distributed throughout the Caribbean Sea but was driven to extinction via hunting by 1952.

These are only a few of the recorded extinctions in the past 500 years. The International Union for Conservation of Nature (IUCN) keeps a list of extinct and endangered species called the Red List. The list is not complete, but it describes 380 extinct species of vertebrates after 1500 AD, 86 of which were driven extinct by overhunting or overfishing.

Estimates of Present-Time Extinction Rates

Estimates of extinction rates are hampered by the fact that most extinctions are probably happening without observation. The extinction of a bird or mammal is likely to be noticed by humans, especially if it has been hunted or used in some other way. But there are many organisms that are of less interest to humans (not necessarily of less value) and many that are undescribed.

The background extinction rate is estimated to be about one per million species per year (E/MSY). For example, assuming there are about ten million species in existence, the expectation is that ten species would become extinct each year (each year represents ten million species per year).

One contemporary extinction rate estimate uses the extinctions in the written record since the year 1500. For birds alone this method yields an estimate of 26 E/MSY. However, this value may be an underestimate for three reasons. First, many species would not have been described until much later in the time period, so their loss would have gone unnoticed. Second, the number of recently extinct vertebrate species is increasing because extinct species now are being described from skeletal remains. And third, some species are probably already extinct even though conservationists are reluctant to name them as such. Taking these factors into account raises the estimated extinction rate closer to 100 E/MSY. The predicted rate by the end of the century is 1500 E/MSY.

A second approach to estimating present-time extinction rates is to correlate species loss with habitat loss by measuring forest-area loss and understanding species-area relationships. The species-area relationship is the rate at which new species are seen when the area surveyed is increased. Studies have shown that the number of species present increases as the size of the island increases. This phenomenon has also been shown to hold true in other island-like habitats as well, such as the mountain-top tepuis of Venezuela, which are surrounded by tropical forest. Turning this relationship around, if the habitat area is reduced, the number of species living there will also decline. Estimates of extinction rates based on habitat loss and species-area relationships have suggested that with about 90 percent habitat loss an expected 50 percent of species would become extinct. Species-area estimates have led to species extinction rate calculations of about 1000 E/MSY and higher. In general, actual observations do not show this amount of loss and suggestions have been made that there is a delay in extinction. Recent work has also called into question the applicability of the species-area relationship when estimating the loss of species. This work argues that the species-area relationship leads to an overestimate of extinction rates. A better relationship to use may be the endemics-area relationship. Using this method would bring estimates down to around 500 E/MSY in the coming century. Note that this value is still 500 times the background rate.

Figure 47.7 Studies have shown that the number of species present increases with the size of the habitat. (credit: modification of work by Adam B. Smith)

Link to Learning

Link to Learning

Check out this interactive exploration of endangered and extinct species, their ecosystems, and the causes of the endangerment or extinction.

Footnotes

• 1Mora Camilo et al., “How Many Species Are There on Earth and in the Ocean?” PLoS Biology (2011), doi:10.1371/journal.pbio.1001127.
• 2Arthur D. Chapman, Numbers of Living Species in Australia and the World, 2nd ed. (Canberra, AU: Australian Biological Resources Study, 2009). www.environment.gov.au/system/files/pages/2ee3f4a1-f130-465b-9c7a-79373680a067/files/nlsaw-2nd-complete.pdf/.
• 3Brian Groombridge and Martin D. Jenkins. World Atlas of Biodiversity: Earth’s Living Resources in the 21^st Century. Berkeley: University of California Press, 2002.
• 4International Institute for Species Exploration (IISE), 2011 State of Observed Species (SOS). Tempe, AZ: IISE, 2011. Accessed May, 20, 2012. http://www.esf.edu/species/.