The text discusses RNA-dependent polymerization, focusing on RNA-dependent RNA polymerases (RdRp) and reverse transcriptases (RNA-dependent DNA polymerases). It explores their roles in viral genome re...The text discusses RNA-dependent polymerization, focusing on RNA-dependent RNA polymerases (RdRp) and reverse transcriptases (RNA-dependent DNA polymerases). It explores their roles in viral genome replication and retrotransposition, explaining the mechanisms of RNA synthesis by RdRp, structure and function of viral polymerases, challenges in fidelity during replication, and the evolutionary implications of error-prone replication.
The text provides an in-depth explanation of DNA microarrays, a technology used to measure gene expression levels or genotype different regions of a genome. DNA microarrays consist of microscopic spot...The text provides an in-depth explanation of DNA microarrays, a technology used to measure gene expression levels or genotype different regions of a genome. DNA microarrays consist of microscopic spots of DNA sequences on a solid surface, functioning as probes that hybridize with target samples under study. The hybridization is detected via labeled targets and helps determine the abundance of nucleic acid sequences. Two types of arrays are highlighted: solid-phase and bead arrays.
