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- https://bio.libretexts.org/Courses/New_England_College/Microbiology_with_NEC/09%3A_Modern_Applications_of_Microbial_Genetics/9.02%3A_Visualizing_and_Characterizing_DNAFinding a gene of interest within a sample requires the use of a single-stranded DNA probe labeled with a molecular beacon (typically radioactivity or fluorescence) that can hybridize with a complemen...Finding a gene of interest within a sample requires the use of a single-stranded DNA probe labeled with a molecular beacon (typically radioactivity or fluorescence) that can hybridize with a complementary single-stranded nucleic acid in the sample. Agarose gel electrophoresis allows for the separation of DNA molecules based on size. Restriction fragment length polymorphism (RFLP) analysis allows for the visualization by agarose gel electrophoresis of distinct variants of a DNA sequence.
- https://bio.libretexts.org/Courses/City_College_of_San_Francisco/Introduction_to_Genetics/12%3A_Techniques_of_Molecular_Genetics/12.07%3A__DNA_Analysis-_Blotting_and_HybridizationBands of DNA in an electrophoretic gel form only if most of the DNA molecules are of the same size, such as following a PCR reaction, or restriction digestion of a plasmid. In other situations, such a...Bands of DNA in an electrophoretic gel form only if most of the DNA molecules are of the same size, such as following a PCR reaction, or restriction digestion of a plasmid. In other situations, such as after restriction digestion of chromosomal (genomic) DNA, there will be a large number of variable size fragments in the digest and it will appear as a continuous smear of DNA, rather than distinct bands.
- https://bio.libretexts.org/Bookshelves/Biochemistry/Book%3A_Biochemistry_Free_For_All_(Ahern_Rajagopal_and_Tan)/08%3A_Basic_Techniques/8.04%3A_Detection_identification_and_quantitation_of_specific_nucleic_acids_and_proteinsOne way to detect the presence of a particular nucleic acid or protein is dependent on transferring the separated molecules from the gels onto a membrane made of nitrocellulose or nylon to create a “b...One way to detect the presence of a particular nucleic acid or protein is dependent on transferring the separated molecules from the gels onto a membrane made of nitrocellulose or nylon to create a “blot” and probing for the molecule(s) of interest using reagents that specifically bind to those molecules. The next section will discuss how this can be done for nucleic acids as well as for proteins.
- https://bio.libretexts.org/Bookshelves/Microbiology/Microbiology_(Boundless)/07%3A_Microbial_Genetics/7.25%3A_Molecular_Techniques/7.25C%3A_Northern_BlotsNorthern blots allow investigators to determine messenger RNA molecular weight and sample content.
- https://bio.libretexts.org/Courses/Manchester_Community_College_(MCC)/Remix_of_Openstax%3AMicrobiology_by_Parker_Schneegurt_et_al/10%3A_Modern_Applications_of_Microbial_Genetics/10.02%3A_Visualizing_and_Characterizing_DNAFinding a gene of interest within a sample requires the use of a single-stranded DNA probe labeled with a molecular beacon (typically radioactivity or fluorescence) that can hybridize with a complemen...Finding a gene of interest within a sample requires the use of a single-stranded DNA probe labeled with a molecular beacon (typically radioactivity or fluorescence) that can hybridize with a complementary single-stranded nucleic acid in the sample. Agarose gel electrophoresis allows for the separation of DNA molecules based on size. Restriction fragment length polymorphism (RFLP) analysis allows for the visualization by agarose gel electrophoresis of distinct variants of a DNA sequence.
- https://bio.libretexts.org/Courses/Portland_Community_College/Cascade_Microbiology/10%3A_Modern_Applications_of_Microbial_Genetics/10.2%3A_Visualizing_and_Characterizing_DNAFinding a gene of interest within a sample requires the use of a single-stranded DNA probe labeled with a molecular beacon (typically radioactivity or fluorescence) that can hybridize with a complemen...Finding a gene of interest within a sample requires the use of a single-stranded DNA probe labeled with a molecular beacon (typically radioactivity or fluorescence) that can hybridize with a complementary single-stranded nucleic acid in the sample. Agarose gel electrophoresis allows for the separation of DNA molecules based on size. Restriction fragment length polymorphism (RFLP) analysis allows for the visualization by agarose gel electrophoresis of distinct variants of a DNA sequence.
- https://bio.libretexts.org/Courses/Sacramento_City_College/BIOL_440%3A_General_Microbiology_(Hughes)/08%3A_Week_8/14%3A_Modern_Applications_of_Microbial_Genetics/14.02%3A_Visualizing_and_Characterizing_DNAFinding a gene of interest within a sample requires the use of a single-stranded DNA probe labeled with a molecular beacon (typically radioactivity or fluorescence) that can hybridize with a complemen...Finding a gene of interest within a sample requires the use of a single-stranded DNA probe labeled with a molecular beacon (typically radioactivity or fluorescence) that can hybridize with a complementary single-stranded nucleic acid in the sample. Agarose gel electrophoresis allows for the separation of DNA molecules based on size. Restriction fragment length polymorphism (RFLP) analysis allows for the visualization by agarose gel electrophoresis of distinct variants of a DNA sequence.
- https://bio.libretexts.org/Bookshelves/Biochemistry/Book%3A_Biochemistry_Free_and_Easy_(Ahern_and_Rajagopal)/09%3A_Techniques/9.11%3A_BlottingSecond, after the gel run is complete, the proteins or nucleic acids in the gel are transferred out of the gel onto a membrane/paper that physically binds to the molecules. The secondary antibody is u...Second, after the gel run is complete, the proteins or nucleic acids in the gel are transferred out of the gel onto a membrane/paper that physically binds to the molecules. The secondary antibody is usually linked to an enzyme which, in the presence of the right reagent, catalyzes a reaction that produces a signal (color or light) indicating where the antibody is bound.
- https://bio.libretexts.org/Bookshelves/Genetics/Online_Open_Genetics_(Nickle_and_Barrette-Ng)/08%3A_Techniques_of_Molecular_Genetics/8.07%3A__DNA_Analysis-_Blotting_and_HybridizationBands of DNA in an electrophoretic gel form only if most of the DNA molecules are of the same size, such as following a PCR reaction, or restriction digestion of a plasmid. In other situations, such a...Bands of DNA in an electrophoretic gel form only if most of the DNA molecules are of the same size, such as following a PCR reaction, or restriction digestion of a plasmid. In other situations, such as after restriction digestion of chromosomal (genomic) DNA, there will be a large number of variable size fragments in the digest and it will appear as a continuous smear of DNA, rather than distinct bands.
- https://bio.libretexts.org/Bookshelves/Genetics/Working_with_Molecular_Genetics_(Hardison)/Unit_I%3A_Genes_Nucleic_Acids_Genomes_and_Chromosomes/3%3A_Isolating_and_Analyzing_Genes/3.10%3A_Functional_analysis_of_isolated_genesIdeally, the primers are in different exons so that the product of amplifying the cDNA will be smaller than the product of amplifying the genomic DNA. In complementary approaches, the labeled DNA can ...Ideally, the primers are in different exons so that the product of amplifying the cDNA will be smaller than the product of amplifying the genomic DNA. In complementary approaches, the labeled DNA can be hybridized in situto thin sections of a tissue or embryo or other specimen, and the resulting pattern of grains visualized along the specimen in the microscope (Figure 3.35).
- https://bio.libretexts.org/Courses/Ohio_State_University/Ohio_State_University_SP22%3A_Molecular_Genetics_4606_(Chamberlin)/13%3A_Detecting_Genes_and_Gene_Products/13.01%3A__DNA_Analysis-_Blotting_and_HybridizationBands of DNA in an electrophoretic gel form only if most of the DNA molecules are of the same size, such as following a PCR reaction, or restriction digestion of a plasmid. In other situations, such a...Bands of DNA in an electrophoretic gel form only if most of the DNA molecules are of the same size, such as following a PCR reaction, or restriction digestion of a plasmid. In other situations, such as after restriction digestion of chromosomal (genomic) DNA, there will be a large number of variable size fragments in the digest and it will appear as a continuous smear of DNA, rather than distinct bands.
