The methylase encoded by the dam gene (dam methylase) transfers a methyl group from SAM to the N 6 position of the adenine base in the sequence 5' … GATC … 3'. Catalyzes the transfer and exchange of a...The methylase encoded by the dam gene (dam methylase) transfers a methyl group from SAM to the N 6 position of the adenine base in the sequence 5' … GATC … 3'. Catalyzes the transfer and exchange of a phosphate group from the g position of rATP (adenine ribose triphosphate nucleotide) to the 5' hydroxyl terminus of double stranded and single stranded DNA or RNA, and nucleoside 3' monophosphates.
Of all the enzymatic functions needed for replication of DNA, the ability to catalyze the incorporation of deoxynucleotides into DNA is most central. Enzymes that catalyze this reaction, DNA polymeras...Of all the enzymatic functions needed for replication of DNA, the ability to catalyze the incorporation of deoxynucleotides into DNA is most central. Enzymes that catalyze this reaction, DNA polymerases, have been isolated from many species, and many species have multiple DNA polymerases. Our earliest and most complete understanding of the mechanism of these enzymes comes from studies of the first DNA polymerase isolated, called DNA polymerase I.
