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- https://bio.libretexts.org/Courses/North_Carolina_State_University/MB352_General_Microbiology_Laboratory_2021_(Lee)/06%3A_Microbial_Physiology/6.02%3A_Temperature_pH_and_Osmotic_RequirementsOne excellent example of this are the Lactic Acid Bacteria (LAB); a large and diverse group of Gram-positive bacteria that produce lactic acid as the major end product of the fermentation of carbohydr...One excellent example of this are the Lactic Acid Bacteria (LAB); a large and diverse group of Gram-positive bacteria that produce lactic acid as the major end product of the fermentation of carbohydrates. The ability to control the movement of water across a membrane is necessary for the survival of all cells. Osmotic pressure is the minimum pressure which needs to be applied to a solution to prevent the inward flow of water across a semi-permeable membrane.
- https://bio.libretexts.org/Courses/North_Carolina_State_University/MB352_General_Microbiology_Laboratory_2021_(Lee)/06%3A_Microbial_Physiology/6.01%3A_Introduction_to_Oxygen_RequirementsThe bottom of the tube of medium is kept anaerobic by cystine and thioglycollic acid, which chemically react with, and tie up any oxygen that diffuses in. The oxygen content of the thioglycollate medi...The bottom of the tube of medium is kept anaerobic by cystine and thioglycollic acid, which chemically react with, and tie up any oxygen that diffuses in. The oxygen content of the thioglycollate medium decreases with depth until the medium becomes anaerobic towards the bottom of the tube. A palladium catalyst in the chamber of the GasPak system catalyzes the formation of water from hydrogen and oxygen, thereby removing oxygen from the sealed chamber (image 3 and 4).
- https://bio.libretexts.org/Courses/North_Carolina_State_University/MB352_General_Microbiology_Laboratory_2021_(Lee)/05%3A_Enumeration_of_Bacteria/5.02%3A_Lab_Procedures-_How_to_operate_a_PipettorRotate the volume adjustment knob until the digital indicator reaches the desired volume, then place a disposable tip on the shaft of the pipette (practice all three volumes min, int, max) Press down ...Rotate the volume adjustment knob until the digital indicator reaches the desired volume, then place a disposable tip on the shaft of the pipette (practice all three volumes min, int, max) Press down on the plunger to the First Stop. (You will be able to push past this point, but there is enough resistance to stop the movement if you try to be aware of it.) To dispense the sample: place the tip against the side wall of the receiving tube and push the plunger down to the first stop.
- https://bio.libretexts.org/Workbench/Clinical_Microbiology_Lab_Manual/07%3A_Environmental_Conditions_and_MicropipettingRotate the volume adjustment knob until the digital indicator reaches the desired volume, then place a disposable tip on the shaft of the pipette (practice all three volumes min, int, max) Press down ...Rotate the volume adjustment knob until the digital indicator reaches the desired volume, then place a disposable tip on the shaft of the pipette (practice all three volumes min, int, max) Press down on the plunger to the First Stop. (You will be able to push past this point, but there is enough resistance to stop the movement if you try to be aware of it.) To dispense the sample: place the tip against the side wall of the receiving tube and push the plunger down to the first stop.
- https://bio.libretexts.org/Learning_Objects/Laboratory_Experiments/Microbiology_Labs/Book%3A_General_Microbiology_Lab_Manual_(Pakpour_and_Horgan)/Lab_05%3A_Pipette_and_Environmental_RequirementsRotate the volume adjustment knob until the digital indicator reaches the desired volume, then place a disposable tip on the shaft of the pipette (practice all three volumes min, int, max) To dispense...Rotate the volume adjustment knob until the digital indicator reaches the desired volume, then place a disposable tip on the shaft of the pipette (practice all three volumes min, int, max) To dispense the sample: place the tip against the side wall of the receiving tube and push the plunger down to the first stop. Once you are finished add your data to the chart at the front of the class, you will need to copy the data for the entire class.
