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- https://bio.libretexts.org/Bookshelves/Introductory_and_General_Biology/General_Biology_2e_(OpenStax)/03%3A_Unit_III-_Genetics/3.07%3A_Biotechnology_and_Genomics/3.7.02%3A_BiotechnologyBiotechnology is the use of biological agents for technological advancement. Biotechnology was used for breeding livestock and crops long before the scientific basis of these techniques was understood...Biotechnology is the use of biological agents for technological advancement. Biotechnology was used for breeding livestock and crops long before the scientific basis of these techniques was understood. Biotechnology has grown rapidly through both academic research and private companies. The primary applications of this technology are in medicine (production of vaccines and antibiotics) and agriculture (genetic modification of crops, such as to increase yields).
- https://bio.libretexts.org/Courses/New_England_College/Microbiology_with_NEC/09%3A_Modern_Applications_of_Microbial_Genetics/9.02%3A_Visualizing_and_Characterizing_DNAFinding a gene of interest within a sample requires the use of a single-stranded DNA probe labeled with a molecular beacon (typically radioactivity or fluorescence) that can hybridize with a complemen...Finding a gene of interest within a sample requires the use of a single-stranded DNA probe labeled with a molecular beacon (typically radioactivity or fluorescence) that can hybridize with a complementary single-stranded nucleic acid in the sample. Agarose gel electrophoresis allows for the separation of DNA molecules based on size. Restriction fragment length polymorphism (RFLP) analysis allows for the visualization by agarose gel electrophoresis of distinct variants of a DNA sequence.
- https://bio.libretexts.org/Courses/San_Diego_State_University/BT@SDSU/02%3A_Resources_and_Reading_Materials/2.04%3A_Genetics_Agriculture_and_Biotechnology_(Suza_and_Lee)/2.4.04%3A_PCR_and_Gel_ElectrophoresisThe polymerase chain reaction laboratory technique is used in a variety of applications to make copies of a specific DNA sequence. This lesson describes how a PCR reaction works, what it accomplishes,...The polymerase chain reaction laboratory technique is used in a variety of applications to make copies of a specific DNA sequence. This lesson describes how a PCR reaction works, what it accomplishes, and its basic requirements for success. Examples of interpreting results are given. PCR’s strengths, weaknesses, and applications to plant biotechnology are explained.
- https://bio.libretexts.org/Bookshelves/Biochemistry/Supplemental_Modules_(Biochemistry)/3._Biotechnology_1/3.3%3A_Polymerase_Chain_Reaction_(PCR)_and_Cloning_of_PCR_ProductsThe length of time of the primer extension steps can be increased if the region of DNA to be amplified is long, however, for the majority of PCR experiments an extension time of 2 minutes is sufficien...The length of time of the primer extension steps can be increased if the region of DNA to be amplified is long, however, for the majority of PCR experiments an extension time of 2 minutes is sufficient to get complete extension. The primary difference is that instead of the primer containing the entire restriction site sequence (say the six nucleotides of a six cutter) it will contain only the last three (and the other PCR primer will contain the complementary sequence for the first three).
- https://bio.libretexts.org/Workbench/BIOL_190%3A_General_Biology_(OpenStax)/03%3A_Unit_III-_Genetics/3.07%3A_Biotechnology_and_Genomics/3.7.02%3A_BiotechnologyBiotechnology is the use of biological agents for technological advancement. Biotechnology was used for breeding livestock and crops long before the scientific basis of these techniques was understood...Biotechnology is the use of biological agents for technological advancement. Biotechnology was used for breeding livestock and crops long before the scientific basis of these techniques was understood. Biotechnology has grown rapidly through both academic research and private companies. The primary applications of this technology are in medicine (production of vaccines and antibiotics) and agriculture (genetic modification of crops, such as to increase yields).
- https://bio.libretexts.org/Bookshelves/Introductory_and_General_Biology/General_Biology_1e_(OpenStax)/3%3A_Genetics/17%3A_Biotechnology_and_Genomics/17.1%3A_BiotechnologyBiotechnology is the use of biological agents for technological advancement. Biotechnology was used for breeding livestock and crops long before the scientific basis of these techniques was understood...Biotechnology is the use of biological agents for technological advancement. Biotechnology was used for breeding livestock and crops long before the scientific basis of these techniques was understood. Biotechnology has grown rapidly through both academic research and private companies. The primary applications of this technology are in medicine (production of vaccines and antibiotics) and agriculture (genetic modification of crops, such as to increase yields).
- https://bio.libretexts.org/Courses/Minnesota_State_Community_and_Technical_College/Biology_of_Human_Concerns_(Daniels)/10%3A_DNA_and_Protein_Synthesis/10.08%3A_BiotechnologyBiotechnology is the use of technology to change the genetic makeup of living things for human purposes. Generally, the goal of biotechnology is to modify organisms so they are more useful to humans. ...Biotechnology is the use of technology to change the genetic makeup of living things for human purposes. Generally, the goal of biotechnology is to modify organisms so they are more useful to humans. For example, biotechnology may be used to create crops that yield more food or resist insect pests or viruses, such as the virus-resistant potatoes pictured above. Research is also underway to use biotechnology to cure human genetic disorders with gene therapy.
- https://bio.libretexts.org/Courses/American_River_College/BIOL_400%3A_Principles_of_Biology_(Wolfe)/04%3A_Untitled_Chapter_4/18%3A_Biotechnology_and_Genomics/18.01%3A_BiotechnologyBiotechnology is the use of biological agents for technological advancement. Biotechnology was used for breeding livestock and crops long before the scientific basis of these techniques was understood...Biotechnology is the use of biological agents for technological advancement. Biotechnology was used for breeding livestock and crops long before the scientific basis of these techniques was understood. Biotechnology has grown rapidly through both academic research and private companies. The primary applications of this technology are in medicine (production of vaccines and antibiotics) and agriculture (genetic modification of crops, such as to increase yields).
- https://bio.libretexts.org/Bookshelves/Genetics/Genetics_Agriculture_and_Biotechnology_(Suza_and_Lee)/01%3A_Chapters/1.04%3A_PCR_and_Gel_ElectrophoresisThe polymerase chain reaction laboratory technique is used in a variety of applications to make copies of a specific DNA sequence. This lesson describes how a PCR reaction works, what it accomplishes,...The polymerase chain reaction laboratory technique is used in a variety of applications to make copies of a specific DNA sequence. This lesson describes how a PCR reaction works, what it accomplishes, and its basic requirements for success. Examples of interpreting results are given. PCR’s strengths, weaknesses, and applications to plant biotechnology are explained.
- https://bio.libretexts.org/Bookshelves/Introductory_and_General_Biology/Biology_(Kimball)/05%3A_DNA/5.12%3A_PyrosequencingThis page discusses efforts by scientists to sequence genomes of different organisms to better understand evolution, microbial diversity, and human disease genetics. While traditional DNA sequencing i...This page discusses efforts by scientists to sequence genomes of different organisms to better understand evolution, microbial diversity, and human disease genetics. While traditional DNA sequencing is common, innovations like pyrosequencing offer faster and more cost-effective alternatives. Pyrosequencing works by fragmenting DNA, replicating it on beads, and using light emissions to identify sequences, achieving the capability to process 20 million base pairs in six hours.
- https://bio.libretexts.org/Bookshelves/Introductory_and_General_Biology/Biology_(Kimball)/11%3A_Genomics/11.02%3A_Polymerase_Chain_ReactionThis page describes the polymerase chain reaction (PCR), a rapid DNA cloning method that involves synthesizing primers, heat-induced strand separation, and polymerization with heat-stable DNA polymera...This page describes the polymerase chain reaction (PCR), a rapid DNA cloning method that involves synthesizing primers, heat-induced strand separation, and polymerization with heat-stable DNA polymerase. Each cycle doubles the DNA amount, enabling exponential amplification, which can produce billions of copies quickly through automation. PCR is effective for analyzing limited DNA samples but is sensitive to contamination.
