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- https://bio.libretexts.org/Bookshelves/Biochemistry/Supplemental_Modules_(Biochemistry)/1%3A_DNA/1.4%3A_DNA_Modifying_EnzymesThe methylase encoded by the dam gene (dam methylase) transfers a methyl group from SAM to the N 6 position of the adenine base in the sequence 5' … GATC … 3'. Catalyzes the transfer and exchange of a...The methylase encoded by the dam gene (dam methylase) transfers a methyl group from SAM to the N 6 position of the adenine base in the sequence 5' … GATC … 3'. Catalyzes the transfer and exchange of a phosphate group from the g position of rATP (adenine ribose triphosphate nucleotide) to the 5' hydroxyl terminus of double stranded and single stranded DNA or RNA, and nucleoside 3' monophosphates.
- https://bio.libretexts.org/Courses/Ohio_State_University/Ohio_State_University_SP22%3A_Molecular_Genetics_4606_(Chamberlin)/11%3A_Amplifying_and_Manipulating_DNA_Fragments/11.02%3A_Isolating_Genomic_DNADNA purification strategies rely on the chemical properties of DNA that distinguish it from other molecules in the cell, namely that it is a very long, negatively charged molecule. To extract purified...DNA purification strategies rely on the chemical properties of DNA that distinguish it from other molecules in the cell, namely that it is a very long, negatively charged molecule. To extract purified DNA from a tissue sample, cells are broken open by grinding or lysing in a solution that contains chemicals that protect the DNA while disrupting other components of the cell (Figure 8.2). These chemicals may include detergents, which dissolve lipid membranes and denature proteins.
- https://bio.libretexts.org/Bookshelves/Genetics/Online_Open_Genetics_(Nickle_and_Barrette-Ng)/08%3A_Techniques_of_Molecular_Genetics/8.02%3A_Isolating_Genomic_DNADNA purification strategies rely on the chemical properties of DNA that distinguish it from other molecules in the cell, namely that it is a very long, negatively charged molecule. To extract purified...DNA purification strategies rely on the chemical properties of DNA that distinguish it from other molecules in the cell, namely that it is a very long, negatively charged molecule. To extract purified DNA from a tissue sample, cells are broken open by grinding or lysing in a solution that contains chemicals that protect the DNA while disrupting other components of the cell (Figure 8.2). These chemicals may include detergents, which dissolve lipid membranes and denature proteins.
- https://bio.libretexts.org/Courses/City_College_of_San_Francisco/Introduction_to_Genetics/12%3A_Techniques_of_Molecular_Genetics/12.02%3A_Isolating_Genomic_DNADNA purification strategies rely on the chemical properties of DNA that distinguish it from other molecules in the cell, namely that it is a very long, negatively charged molecule. To extract purified...DNA purification strategies rely on the chemical properties of DNA that distinguish it from other molecules in the cell, namely that it is a very long, negatively charged molecule. To extract purified DNA from a tissue sample, cells are broken open by grinding or lysing in a solution that contains chemicals that protect the DNA while disrupting other components of the cell (Figure 8.2). These chemicals may include detergents, which dissolve lipid membranes and denature proteins.
- https://bio.libretexts.org/Bookshelves/Genetics/Working_with_Molecular_Genetics_(Hardison)/Unit_II%3A_Replication_Maintenance_and_Alteration_of_the_Genetic_Material/5._DNA_replication_I%3A_Enzymes_and_mechanism/PolymerasesOf all the enzymatic functions needed for replication of DNA, the ability to catalyze the incorporation of deoxynucleotides into DNA is most central. Enzymes that catalyze this reaction, DNA polymeras...Of all the enzymatic functions needed for replication of DNA, the ability to catalyze the incorporation of deoxynucleotides into DNA is most central. Enzymes that catalyze this reaction, DNA polymerases, have been isolated from many species, and many species have multiple DNA polymerases. Our earliest and most complete understanding of the mechanism of these enzymes comes from studies of the first DNA polymerase isolated, called DNA polymerase I.
