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- https://bio.libretexts.org/Courses/North_Central_State_College/BIOL_1550%3A_Microbiology_(2025)/26%3A_Laboratory_Analysis_of_the_Immune_Response/26.04%3A_Enzyme_Immunoassays_(EIA)_and_Enzyme-Linked_Immunosorbent_Assays_(ELISA)Enzyme immunoassays (EIA) are used to visualize and quantify antigens. They use an antibody conjugated to an enzyme to bind the antigen, and the enzyme converts a substrate into an observable end prod...Enzyme immunoassays (EIA) are used to visualize and quantify antigens. They use an antibody conjugated to an enzyme to bind the antigen, and the enzyme converts a substrate into an observable end product. The substrate may be either a chromogen or a fluorogen. Immunostaining is an EIA technique for visualizing cells in a tissue (immunohistochemistry) or examining intracellular structures (immunocytochemistry). Direct ELISA is used to quantify an antigen in solution.
- https://bio.libretexts.org/Bookshelves/Biochemistry/Fundamentals_of_Biochemistry_(Jakubowski_and_Flatt)/01%3A_Unit_I-_Structure_and_Catalysis/05%3A_Protein_Function/5.07%3A__Binding_-_Enzyme_Linked_Immunosorbant_Assays_(ELISAs)The page provides an educational outline for junior and senior biochemistry majors focused on understanding and applying ELISAs (Enzyme-Linked Immunosorbent Assays). It details the fundamental princip...The page provides an educational outline for junior and senior biochemistry majors focused on understanding and applying ELISAs (Enzyme-Linked Immunosorbent Assays). It details the fundamental principles, distinguishing between formats, key steps in protocols, detection methods, interpretation of results, and advanced techniques like logit-log transformations for data analysis.
- https://bio.libretexts.org/Bookshelves/Microbiology/Microbiology_(OpenStax)/20%3A_Laboratory_Analysis_of_the_Immune_Response/20.04%3A_Enzyme_Immunoassays_(EIA)_and_Enzyme-Linked_Immunosorbent_Assays_(ELISA)Enzyme immunoassays (EIA) are used to visualize and quantify antigens. They use an antibody conjugated to an enzyme to bind the antigen, and the enzyme converts a substrate into an observable end prod...Enzyme immunoassays (EIA) are used to visualize and quantify antigens. They use an antibody conjugated to an enzyme to bind the antigen, and the enzyme converts a substrate into an observable end product. The substrate may be either a chromogen or a fluorogen. Immunostaining is an EIA technique for visualizing cells in a tissue (immunohistochemistry) or examining intracellular structures (immunocytochemistry). Direct ELISA is used to quantify an antigen in solution.
- https://bio.libretexts.org/Courses/Sacramento_City_College/BIOL_440%3A_General_Microbiology_(Hughes)/16%3A_Week_16/27%3A_Laboratory_Analysis_of_the_Immune_Response/27.04%3A_Enzyme_Immunoassays_(EIA)_and_Enzyme-Linked_Immunosorbent_Assays_(ELISA)Enzyme immunoassays (EIA) are used to visualize and quantify antigens. They use an antibody conjugated to an enzyme to bind the antigen, and the enzyme converts a substrate into an observable end prod...Enzyme immunoassays (EIA) are used to visualize and quantify antigens. They use an antibody conjugated to an enzyme to bind the antigen, and the enzyme converts a substrate into an observable end product. The substrate may be either a chromogen or a fluorogen. Immunostaining is an EIA technique for visualizing cells in a tissue (immunohistochemistry) or examining intracellular structures (immunocytochemistry). Direct ELISA is used to quantify an antigen in solution.
- https://bio.libretexts.org/Courses/City_College_of_San_Francisco/Introduction_to_Microbiology_(Liu_et_al.)/26%3A_Diagnosing_Infections/26.05%3A_Enzyme_Immunoassays_(EIA)_and_Enzyme-Linked_Immunosorbent_Assays_(ELISA)This page details various immunoassay techniques, emphasizing enzyme immunoassays (EIAs) and enzyme-linked immunosorbent assays (ELISAs) for antigen and antibody detection. It covers different ELISA m...This page details various immunoassay techniques, emphasizing enzyme immunoassays (EIAs) and enzyme-linked immunosorbent assays (ELISAs) for antigen and antibody detection. It covers different ELISA methods (direct, indirect, sandwich) and other techniques such as immunohistochemistry. Additionally, it addresses MMR titer tests for healthcare workers' immunity verification.
