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- https://bio.libretexts.org/Courses/West_Los_Angeles_College/Biotechnology/04%3A_Genetic_Engineering_and_Recombinant_DNA_Technology/4.01%3A_Principles_of_Genetic_EngineeringGenetic engineering is at the heart of biotechnology. Through the manipulation of an organism's DNA in the laboratory, researchers can add, remove, or change specific genes in an organism, producing ...Genetic engineering is at the heart of biotechnology. Through the manipulation of an organism's DNA in the laboratory, researchers can add, remove, or change specific genes in an organism, producing highly desired traits. This page summarizes the major principles and tools of genetic engineering, in addition to outlining the foundational technique of cloning genes into plasmids and the production of recombinant plasmids.
- https://bio.libretexts.org/Bookshelves/Introductory_and_General_Biology/Map%3A_Raven_Biology_12th_Edition/17%3A_Biotechnology/17.01%3A_Recombinant_DNA/17.1.03%3A_Introducting_Recombinant_DNA_into_Host_CellsStep I: The DNA of a bacterial cell is located in the cytoplasm (1), but also in the plasmid, an independent, circular loop of DNA. The gene to be transferred (4) is located on the plasmid of cell 1 (...Step I: The DNA of a bacterial cell is located in the cytoplasm (1), but also in the plasmid, an independent, circular loop of DNA. The gene to be transferred (4) is located on the plasmid of cell 1 (3), but not on the plasmid of bacterial cell 2 (2). Hence one can easily obtain either double‑ or single‑stranded forms of thes plasmids. {The "blue" comes from the blue‑white screening for recombinants that can be done when the multiple cloning sites are in the b‑galactosidase gene.
- https://bio.libretexts.org/Bookshelves/Genetics/Working_with_Molecular_Genetics_(Hardison)/Unit_I%3A_Genes_Nucleic_Acids_Genomes_and_Chromosomes/3%3A_Isolating_and_Analyzing_Genes/3.04%3A_Introducting_Recombinant_DNA_into_Host_CellsStep I: The DNA of a bacterial cell is located in the cytoplasm (1), but also in the plasmid, an independent, circular loop of DNA. The gene to be transferred (4) is located on the plasmid of cell 1 (...Step I: The DNA of a bacterial cell is located in the cytoplasm (1), but also in the plasmid, an independent, circular loop of DNA. The gene to be transferred (4) is located on the plasmid of cell 1 (3), but not on the plasmid of bacterial cell 2 (2). Hence one can easily obtain either double‑ or single‑stranded forms of thes plasmids. {The "blue" comes from the blue‑white screening for recombinants that can be done when the multiple cloning sites are in the b‑galactosidase gene.
