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Cph1

  • Page ID
    4436
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    Class: Cyanobacteriochrome

    Family: Red/Green

    Origin: Synechocystis

    Chromophore(s): PCB

    Cph1abs.jpghttp://larsenlab.ucdavis.edu/@api/deki/files/1602/Cph1.txt

    Cph1abs.PNG

    Red illumination of the dark adapted 15Z Pr state of Cph1Δ (Figure 1B, red curve) initiates forward photoconversion (Pr to Pfr), generating the primary isomerized Lumi-Rf intermediate. Lumi-Rf thermally evolves via several intermediates to generate the 15E Pfr photoproduct (Figure 1B, dark red curve) on a >100 ms timescale. The dark-stable 15Z Pr state can be regenerated from 15E Pfr either rapidly by far-red light (∼700 nm) or via spontaneous dark reversion on a very slow (>24 h) timescale.

    Cph1photocycle.PNG

    References

    1. Heterogeneous Photodynamics of the Pfr State in the Cyanobacterial Phytochrome Cph1, Peter W. Kim, Nathan C. Rockwell, Shelley S. Martin, J. Clark Lagarias, Delmar S. Larsen, Biochemistry, 53 (28), pp 4601–4611 (2014). pdf
    2. Dynamic Inhomogeneity in the Cyanobacterial Phytochrome Cph1, Peter W. Kim, Nathan C. Rockwell, Shelley S. Martin, J. Clark Lagarias, Delmar S. Larsen, Biochemistry 53, 2818–2826 (2014). pdf
    3. Unraveling the Primary Isomerization Dynamics of the Cph1 Cyanobacterial Phytochrome with Multi-pulse Manipulations, Peter W. Kim, Nathan Rockwell, Lucy Freer, Che-Wei Chang, Shelley Martin, J. Clark Lagarias, Delmar S. Larsen, J. Phys. Chem. Lett., 4, pp 2605–2609 (2013). pdf
    4. Ultrafast E to Z Photoisomerization Dynamics of the Cph1 Phytochrome, Peter W. Kim, Jie Pan, Nathan C. Rockwell, Che-Wei Chang, Keenan C. Taylor, J. Clark Lagarias, Delmar S. Larsen, Chem. Phys. Letters, 549, 86–92 (2012). pdf

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