2.1: Microbiology and Protista Lab
- Page ID
- 24631
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Learning Objectives
After completing this lab, student should be able to:
- Describe the basic structures of a bacterial cell.
- State the three domains of life.
- Name the shape of a given bacteria specimen.
- State the domain of cyanobacteria.
- Be able to identify the cyanobacteria examples viewed in the lab.
- State the domain of the protista.
- Be able to identify the green algae examples viewed in the lab and know if they are colonial or filamentous.
- Be able to recognize the protista specimen viewed in the lab.
- Identify protista as photosynthetic or heterotrophic.
Lab 2: Microbiology from Lumen Learning
Download a PDF of the lab to print.
Part 1: Prokaryotes
Procedure:
- Access the page “Reading: Prokaryotes.”
- We will not be using any live bacteria specimens. Instead, watch this video about aseptic technique. This technique is important to avoid microorganism contamination.
Questions:
- Answer the questions below based on the video.
- What two tools are most commonly used to transfer bacteria?_______________________________________________________________________________________
- With the Bunsen burner, what color is the hottest flame?___________________________________________________________________________________________
- How are the inoculation tools sterilized?_________________________________________________________________________________________________________
- When transferring bacteria from a liquid culture to a Petri plate, why do you turn the plate while spreading the bacteria?________________________________________ _________________________________________________________________________________________________________________________________________
- When transferring bacteria from a Petri plate to a stab culture, how many times should you stab the needle?_________________________________________________
- When transferring bacteria into a liquid tube do you flame the mouth of the tube before inoculation, after inoculation, or both?__________________________________
- Skip to the end of the lab activity where it says “Prepared slides of typical bacteria” and view the prepared slides of bacterial shapes available in the laboratory.
1. Draw a picture of the coccus shaped bacteria.
2. Draw a picture of the bacillus shaped bacteria.
3. Draw a picture of the spirillum shaped bacteria.
3. View the prepared slides of cyanobacteria available in the laboratory. Although they are single-celled, note how they form colonies and attach to one another.
- What is the function of the heterocyst in the Anabaena?_________________________________________________________________________________________ ______________________________________________________________________________________________________________________________________
- If the Oscillatoria is moving, describe the movement quality below.________________________________________________________________________________ ______________________________________________________________________________________________________________________________________
- Which cyanobacteria species form chains? Which cyanobacteria species form clumps?_________________________________________________________________ ______________________________________________________________________________________________________________________________________
Part 2: Protista
Procedure:
- Access the page “Reading: Protists.”
- Watch this video.
Questions:
- View the Euglenozoans specimens available.
- What color is the euglena?__________________________________________________________________________________________________________________
- What structure does the euglena use to move?__________________________________________________________________________________________________
- Can you see any internal chloroplasts?_________________________________________________________________________________________________________
- Can you see the red eyespot? It does not give the organism vision, rather allows it to sense the presence of light.____________________________________________
- Trypanosoma sp. cause African sleeping sickness. (This disease was discussed in the video.) What part of the human body does the Trypanosoma invade?___________ ________________________________________________________________________________________________________________________________________
- What structure does the Trypanosoma use to move?______________________________________________________________________________________________
- How does the Trypanosoma avoid being killed by the white blood cells?_______________________________________________________________________________
- Can African sleeping sickness cause death?______________________________________________________________________________________________________
- View the diatom specimens available.
- What material is found in the cell wall of the diatoms?_____________________________________________________________________________________________
- Are the organisms single or multicellular?________________________________________________________________________________________________________
- View the brown algae specimens available.
- What pigment do brown algae use for photosynthesis?_____________________________________________________________________________________________
- Name and describe the characteristics of one brown algae specimen below.____________________________________________________________________________ _________________________________________________________________________________________________________________________________________
- View the dinoflagellate specimens available.
- What structure does the dinoflagellate use for movement? How many of these structures does it have?______________________________________________________
- Are the organisms single or multicellular?________________________________________________________________________________________________________
- View the ciliate specimens available.
- What structure does Paramecium use to move? Does it have only one or many of these structures?_________________________________________________________
- Paramecium contains two nuclei, a macronucleus (large) and a micronucleus (small). Can you find both of them on your specimen?_______________________________
- Paramecium also contains contractile vacuoles that help maintain water balance through osmosis. Can you locate any on your specimen?__________________________
- View the red algae specimens available.
- What pigment do red algae use for photosynthesis?_______________________________________________________________________________________________
- Name and describe the characteristics of one red algae specimen below.______________________________________________________________________________
- View the green algae specimens available.
- What pigment do green algae use for photosynthesis?_____________________________________________________________________________________________
- Name and describe the characteristics of one green algae specimen below.____________________________________________________________________________
- View the Tubulinid specimens available.
- What structure does Amoeba use to move?______________________________________________________________________________________________________
- Is the Amoeba single or multicellular?__________________________________________________________________________________________________________
- The Amoeba contains contractile vacuoles that help maintain water balance through osmosis. Can you locate any on your specimen?_____________________________
Summary Questions:
Answer the questions below to summarize the lab activity:
1. What type of cell is considered more primitive or basic?
2. State one difference between a prokaryotic and a eukaryotic cell.
3. What two domains contain prokaryotic celled organisms?
4. Identify structures 1, 3, 4, 5, 6, 7, 8, and 9 on the generalized prokaryotic cell pictured below.
5. Are the cyanobacteria autotrophic or heterotrophic?
6. Which cyanobacteria species secretes a gelatinous sheath?
7. Which protista are most similar to green plants? Why?
8. You viewed several protista that exhibited movement. Give an example of a protista that used each of the following movement structures:
- Flagella:
- Cilia:
- Pseudopod:
9. Give two examples of photosynthetic protista you viewed in lab and state what pigment each uses for photosynthesis.
Licenses and Attributions
CC licensed content, Original
- Biology 102 Labs. Authored by: Lynette Hauser. Provided by: Tidewater Community College. Located at: http://www.tcc.edu/. License: CC BY: Attribution
CC licensed content, Shared previously
- Prokaryotes Lab (Biology 102). Authored by: Michael J. Gregory, Ph.D.. Located at: https://b51ab7d9e5e1e7063dcb70cee5c33cf7f4b7bad8.googledrive.com/host/0Bx6hk6AUBHxDc2d4TDJZTFIyMGs/default.htm. License: CC BY-NC-SA: Attribution-NonCommercial-ShareAlike