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3.5: APPENDIX C2 Primer design worksheet

  • Page ID
    26475
  • Name:__________________________________________________________________

    Group members: __________________________________________________________

    Section/TA name_________________________________________________________

    Point mutation (use numbering from Appendix B2)______________________________

    Please also circle your desired mutation below:
    (688)tcc cccaactacg acaagtggga gatggaacgc
    721 acggacatca ccatgaagca caagctgggc gggggccagt acggggaggt gtacgagggc
    781 gtgtggaaga aatacagcct gacggtggcc gtgaagacct tgaaggagga caccatggag
    841 gtggaagagt tcttgaaaga agctgcagtc atgaaagaga tcaaacaccc taacctggtg
    901 cagctccttg gggtctgcac ccgggagccc ccgttctata tcatcactga gttcatgacc
    961 tacgggaacc tcctggacta cctgagggag tgcaaccggc aggaggtgaa cgccgtggtg
    1021 ctgctgtaca tggccactca gatctcgtca gccatggagt acctggagaa gaaaaacttc
    1081 atccacagag atcttgctgc ccgaaactgc ctggtagggg agaaccactt ggtgaaggta
    1141 gctgattttg gcctgagcag gttgatgaca ggggacacct acacagccca tgctggagcc
    1201 aagttcccca tcaaatggac tgcacccgag agcctggcct acaacaagtt ctccatcaag
    1261 tccgacgtct gggcatttgg agtattgctt tgggaaattg ctacctatgg catgtcccct
    1321 tacccgggaa ttgacctgtc ccaggtgtat gagctgctag agaaggacta ccgcatggag
    1381 cgcccagaag gctgcccaga gaaggtctat gaactcatgc gagcatgttg gcagtggaat
    1441 ccctctgacc ggccctcctt tgctgaaatc caccaagcct ttgaaacaat gttccaggaa
    1501 tccagtatct cagacgaagt ggaaaaggag ctgggg

    Corresponding amino acid substitution (use numbering from Appendix B1)___________

    Forward Primer (5’ to 3’)
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    Reverse Primer (5’ to 3’)
    ________________________________________________________________________

    Percent GC _______________ Tm_______________________________