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2.3.1.8: Transformation Supplies

  • Page ID
    114849
    • Nathan Reyna, Ruth Plymale, & Kristen Johnson
    • Ouachita Babtist University & University of New Hampshire

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    Transformation:

    Materials

    Amount Needed

    (Per Reaction)

    Competent Bacteria Cells

    (JM109= Cat#: T3003)

    25-50 uL

    Ligation Mixture

    1-5 uL

    LB Agar with Antibiotics (either Kan, Chlor, Amp, or Tet)

    1 plate (depending on backbone)

    SOC Media (Cat#: 15544034)

    200 uL

    Sterile Glass Beads

    Approximately 4-8 beads

    Ice Bucket

    1

    Microcentrifuge Tube

    1

    Shaking Incubator (37C)

    1 (used by class)

    Plate Warmer (37C)

    (Can be the same as the Shaking Incubator)

    1 (used by class)

    Incubator

    1 (used by class)


    This page titled 2.3.1.8: Transformation Supplies is shared under a not declared license and was authored, remixed, and/or curated by Nathan Reyna, Ruth Plymale, & Kristen Johnson.

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