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2.3.1.2: Adding Antibiotics

  • Page ID
    114843
    • Nathan Reyna, Ruth Plymale, & Kristen Johnson
    • Ouachita Babtist University & University of New Hampshire

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    *Color coding LB-agar plates is recommended to simplify which plate contains which antibiotic*

    **Addition of antibiotics to media is done after autoclaving, when the media has cooled to 50-55oC**

    Ampicillin (Amp/A): *Working concentration 100 ug/mL freezer stock 100 mg/mL (in water)

    • Add 1 mL of 100 mg/mL ampicillin (dissolved in water) into 1 L of LB broth or agar

    Chloramphenicol (Chlor/C): *Working concentration 35 ug/mL freezer stock 35 mg/mL (in EtOH)

    • Add 1 mL of 35 mg/mL chloramphenicol (dissolved in ethanol) into 1 L of LB broth or agar

    Kanamycin (Kan/K): *Working Concentration 50 ug/mL freezer stock 50 mg/mL (in water)

    • Add 1 mL of 50 mg/mL kanamycin (dissolved in water) into 1 L of LB broth or agar

    Tetracycline (Tet/T): *Working concentration 10 ug/mL freezer stock 10 mg/mL (in water)

    • Add 1 mL of 10 mg/mL tetracycline (dissolved in water) into 1 L LB broth or agar

    This page titled 2.3.1.2: Adding Antibiotics is shared under a not declared license and was authored, remixed, and/or curated by Nathan Reyna, Ruth Plymale, & Kristen Johnson.