Day 5
- Page ID
- 115049
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Advance Preparation
Be sure to watch the videos that review DNA replication, introduce Hi-Fi assembly, and bacterial transformation.
DNA replication: https://www.youtube.com/watch?v=TNKWgcFPHqw&t=207s
Hi-Fi assembly 1: https://www.neb.com/en-us/tools-and-resources/video-library/introduction-to-nebuilder-hifi-dna-assembly?autoplay=1
Hi-Fi assembly 2: https://www.neb.com/en-us/tools-and-resources/video-library/nebuilder-hifi-dna-assembly-bridging-dsdna-with-a-ssdna-oligo?autoplay=1
Hi-Fi assembly 3: https://www.neb.com/en-us/tools-and-resources/video-library/construction-of-an-sgrna-cas9-expression-vector-via-an-ssoligo-bridge?autoplay=1
Bacterial Transformation: https://www.youtube.com/watch?v=w_fZmiIAdco
Activities
Morning:
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Introduction to Hi-Fi assembly.
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Introduction to bacterial transformation.
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Measure chlorophyll autofluorescence and take pictures of plates (Protocols --> Heat Tolerance of seedling elongation and root growth)
Afternoon:
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Dilute Oligos (Protocols --> CRISPR Construct Experiment).
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Set up Hi-Fi Assembly reaction.
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Bacterial transformation


