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10: Modern Applications of Microbial Genetics

Last updated

Jun 23, 2019
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- 9.7: Gene Regulation: Operon Theory
- 10.1: Microbes and the Tools of Genetic Engineering

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Topic hierarchy

10.1: Microbes and the Tools of Genetic Engineering
10.2: Visualizing and Characterizing DNA
Finding a gene of interest within a sample requires the use of a single-stranded DNA probe labeled with a molecular beacon (typically radioactivity or fluorescence) that can hybridize with a complementary single-stranded nucleic acid in the sample. Agarose gel electrophoresis allows for the separation of DNA molecules based on size. Restriction fragment length polymorphism (RFLP) analysis allows for the visualization by agarose gel electrophoresis of distinct variants of a DNA sequence.
10.3: Whole Genome Methods and Industrial Applications
Advances in molecular biology have led to the creation of entirely new fields of science. Among these are fields that study aspects of whole genomes, collectively referred to as whole-genome methods. In this section, we’ll provide a brief overview of the whole-genome fields of genomics, transcriptomics, and proteomics.
10.4: Genetic Engineering - Risks, Benefits, and Perceptions
Many types of genetic engineering have yielded clear benefits with few apparent risks. However, many emerging applications of genetic engineering are much more controversial, often because their potential benefits are pitted against significant risks, real or perceived. This is certainly the case for gene therapy, a clinical application of genetic engineering that may one day provide a cure for many diseases but is still largely an experimental approach to treatment.

Thumbnail: A group of Genetically modified GloFish fluorescent fish. Image used with permission (www.glofish.com).

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