OER - BIOL 2420 Laboratory Manual
- Page ID
- 194111
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\(\newcommand{\avec}{\mathbf a}\) \(\newcommand{\bvec}{\mathbf b}\) \(\newcommand{\cvec}{\mathbf c}\) \(\newcommand{\dvec}{\mathbf d}\) \(\newcommand{\dtil}{\widetilde{\mathbf d}}\) \(\newcommand{\evec}{\mathbf e}\) \(\newcommand{\fvec}{\mathbf f}\) \(\newcommand{\nvec}{\mathbf n}\) \(\newcommand{\pvec}{\mathbf p}\) \(\newcommand{\qvec}{\mathbf q}\) \(\newcommand{\svec}{\mathbf s}\) \(\newcommand{\tvec}{\mathbf t}\) \(\newcommand{\uvec}{\mathbf u}\) \(\newcommand{\vvec}{\mathbf v}\) \(\newcommand{\wvec}{\mathbf w}\) \(\newcommand{\xvec}{\mathbf x}\) \(\newcommand{\yvec}{\mathbf y}\) \(\newcommand{\zvec}{\mathbf z}\) \(\newcommand{\rvec}{\mathbf r}\) \(\newcommand{\mvec}{\mathbf m}\) \(\newcommand{\zerovec}{\mathbf 0}\) \(\newcommand{\onevec}{\mathbf 1}\) \(\newcommand{\real}{\mathbb R}\) \(\newcommand{\twovec}[2]{\left[\begin{array}{r}#1 \\ #2 \end{array}\right]}\) \(\newcommand{\ctwovec}[2]{\left[\begin{array}{c}#1 \\ #2 \end{array}\right]}\) \(\newcommand{\threevec}[3]{\left[\begin{array}{r}#1 \\ #2 \\ #3 \end{array}\right]}\) \(\newcommand{\cthreevec}[3]{\left[\begin{array}{c}#1 \\ #2 \\ #3 \end{array}\right]}\) \(\newcommand{\fourvec}[4]{\left[\begin{array}{r}#1 \\ #2 \\ #3 \\ #4 \end{array}\right]}\) \(\newcommand{\cfourvec}[4]{\left[\begin{array}{c}#1 \\ #2 \\ #3 \\ #4 \end{array}\right]}\) \(\newcommand{\fivevec}[5]{\left[\begin{array}{r}#1 \\ #2 \\ #3 \\ #4 \\ #5 \\ \end{array}\right]}\) \(\newcommand{\cfivevec}[5]{\left[\begin{array}{c}#1 \\ #2 \\ #3 \\ #4 \\ #5 \\ \end{array}\right]}\) \(\newcommand{\mattwo}[4]{\left[\begin{array}{rr}#1 \amp #2 \\ #3 \amp #4 \\ \end{array}\right]}\) \(\newcommand{\laspan}[1]{\text{Span}\{#1\}}\) \(\newcommand{\bcal}{\cal B}\) \(\newcommand{\ccal}{\cal C}\) \(\newcommand{\scal}{\cal S}\) \(\newcommand{\wcal}{\cal W}\) \(\newcommand{\ecal}{\cal E}\) \(\newcommand{\coords}[2]{\left\{#1\right\}_{#2}}\) \(\newcommand{\gray}[1]{\color{gray}{#1}}\) \(\newcommand{\lgray}[1]{\color{lightgray}{#1}}\) \(\newcommand{\rank}{\operatorname{rank}}\) \(\newcommand{\row}{\text{Row}}\) \(\newcommand{\col}{\text{Col}}\) \(\renewcommand{\row}{\text{Row}}\) \(\newcommand{\nul}{\text{Nul}}\) \(\newcommand{\var}{\text{Var}}\) \(\newcommand{\corr}{\text{corr}}\) \(\newcommand{\len}[1]{\left|#1\right|}\) \(\newcommand{\bbar}{\overline{\bvec}}\) \(\newcommand{\bhat}{\widehat{\bvec}}\) \(\newcommand{\bperp}{\bvec^\perp}\) \(\newcommand{\xhat}{\widehat{\xvec}}\) \(\newcommand{\vhat}{\widehat{\vvec}}\) \(\newcommand{\uhat}{\widehat{\uvec}}\) \(\newcommand{\what}{\widehat{\wvec}}\) \(\newcommand{\Sighat}{\widehat{\Sigma}}\) \(\newcommand{\lt}{<}\) \(\newcommand{\gt}{>}\) \(\newcommand{\amp}{&}\) \(\definecolor{fillinmathshade}{gray}{0.9}\)- Front Matter
- This page discusses the LibreTexts platform, which offers customizable textbooks to lower student costs and promotes open educational initiatives. It features a microbiology laboratory manual aimed at non-science majors, aligning with state learning outcomes and under a Creative Commons license. Key exercises cover safety, microscope use, and bacterial identification, highlighting the manual's contributions to microbiology education for allied health careers.
- 1: Section One - Getting Started In The Microbiology Laboratory
- This page introduces essential concepts for safe work in a microbiology lab, focusing on laboratory safety, microscopy, and aseptic techniques for growing microbes. Mastery of these principles is vital for success in future lab activities, with a video provided to highlight their importance for health-care professionals.
- 2: Section Two - Visualizing Bacteria (Staining Bacteria)
- This page discusses common staining techniques in microbiology, highlighting their role in visualizing microbial cells and understanding their structural composition. It also covers bacterial cell structures, shapes, and arrangements, which deepen the comprehension of microbial morphology. A video resource is included for additional learning.
- 2.1: Exercise #6 - Bacterial Cellular Morphology and Structure
- 2.2: Exercise #7 - Specimen Preparation and Simple Staining
- 2.3: Exercise #8 - Differential Staining- Gram Staining
- 2.4: Exercise #9 - Differential Staining- Acid-Fast Stain
- 2.5: Exercise #10 - Structural Stain- Endospore Stain
- 2.6: Exercise #11 - Structural Stain- Capsule Stain
- 2.7: Section Two Review
- 3: Section Three Assessing Metabolic Characteristics of Bacteria (Biochemical Tests, Selective and Differential Media)
- This page discusses microscopy and staining techniques for assessing microbial cell characteristics like shape and size, but notes their limitations in differentiating species within the same genus.
- 3.1: Exercise #12 - Carbohydrate Fermentation Tests
- 3.2: Exercise #13 - Triple Sugar Iron Agar Test
- 3.3: Exercise #14 - SIM Medium Test
- 3.4: Exercise 15 - IMViC Tests
- 3.5: Exercise #16 - Respiration Tests
- 3.6: Exercise #17 - Urease Test
- 3.7: Exercise #18 - Coagulase Test
- 3.8: Exercise #19 - Casease Test
- 3.9: Exercise #20 - Starch Hydrolysis Test
- 3.10: Exercise #21 - Blood Agar
- 3.11: Exercise #22 MacConkey Agar
- 3.12: Exercise #23 - Mannitol Salt Agar
- 3.13: Section Three Review
- 4: Section Four - Enumerating Bacteria
- This page highlights the importance of accurately measuring microbial growth in microbiology, focusing on viable plate counts and direct microscopic counts as primary methods. It emphasizes the need for effective enumeration due to the impracticality of counting each microbial cell individually.
- 5: Section Five - Factors Affecting Microbial Growth
- This page discusses the factors affecting microbial growth, including temperature, pH, and antimicrobial agents, emphasizing the structural differences among microorganisms. It highlights the importance of these factors for optimizing research and controlling microbial growth. Additionally, it covers the Kirby-Bauer method, a crucial clinical test for determining effective antimicrobials for patient treatment.
- 6: Section Six - Eukaryotic Pathogens (A Survey of Fungal, Protozoan, and Helminth Pathogens)
- This page provides an overview of eukaryotic pathogens, including fungi, protozoans, and helminths. It emphasizes their identification via microscopy, covering their scientific and common names, transmission methods, host anatomical locations, and associated diseases. While not comprehensive, it aims to establish a foundational understanding of these pathogens' significance in health and disease.
- Back Matter
- This page provides a detailed glossary of microbiological terms, covering definitions for microorganisms (bacteria, fungi, helminths, protozoans) and related concepts like growth media and staining techniques. It also includes classifications of microorganisms, discusses growth conditions, and outlines essential laboratory techniques such as aseptic practices and culturing methods.