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4.2: Diffusion and Osmosis (Instructor Materials Preparation)

  • Page ID
    34830
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    Lab Materials

    This is the prep for one section of 24 students.

    Part 1: Brownian Motion

    Students will do this part individually.

    Materials Quantity Notes
    Microscope slides 1 per student Also used in Part 3
    Coverslips 1 per student Also used in Part 3
    Water dropper bottles 1 per table
    Carmine red powder 2 small watch glasses On side counter

    Part 2: Diffusion across a Semipermeable Membrane

    Students will do this part in table teams.

    Materials Quantity Notes
    Beakers (400 mL) 2 per table
    Wax pencil 1 per table
    Dental floss or thread 1 per table
    Dialysis tubing 2 per table
    Phenolphthalein 1 per table Dropper bottle—does not go down drain!
    1 M NaOH (sodium hydroxide) 1 per table Dropper bottle
    Iodine (I2KI) 1 per table Dropper bottle
    1% starch solution ~10mL per table On side counter
    Pipette/pipettor setup for starch (10 ml) 2 per class On side counter—specifically for starch
    Pipette/pipettor set up 1–2 per table
    Large waste bucket for starch 1–2 per class

    Part 3: Osmosis and the Cell Membrane

    Students will do this part individually.

    Materials Quantity Notes
    Elodea 2 bowls On side counter
    Forceps 2 pairs per bowl
    5% NaCl solution 1 per table Dropper bottle
    Slidewash bowls 1–2 per room

    Part 4: Experimental Design

    Students will do this part in pairs.

    Materials Quantity Notes
    2% agar plates 2 per table Use deep petri dishes for diffusion experiment
    Clear plastic rulers 2 per table
    Potassium permanganate 1 per table Dropper bottle
    Methylene blue 1 per table Dropper bottle
    Cork borers 2–3 sets On side table

    Contributors and Attributions

    CC licensed content, Original
    • Biology Labs. Authored by: Wendy Riggs. Provided by: College of the Redwood. Located at: www.redwoods.edu. License: CC BY: Attribution

    4.2: Diffusion and Osmosis (Instructor Materials Preparation) is shared under a not declared license and was authored, remixed, and/or curated by LibreTexts.

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