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Biology LibreTexts

11: Spectrophotometry

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Learning Objectives
Content Objectives
  • Identify the main features of the spectrophotometer and describe their functions.
  • Use the spectrophotometer to obtain an absorbance spectrum.
Skill Objectives
  • Identify the parts of a spectrophotometer and their related functions.
  • “Blank” a spectrophotometer.
  • Obtain an absorbance spectrum for a molecule.
  • Use the wavelength absorption scans to determine the dyes in colored skittles.


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