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Biology LibreTexts

11: Spectrophotometry

  • Page ID
    95255
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    Learning Objectives
    Content Objectives
    • Identify the main features of the spectrophotometer and describe their functions.
    • Use the spectrophotometer to obtain an absorbance spectrum.
    Skill Objectives
    • Identify the parts of a spectrophotometer and their related functions.
    • “Blank” a spectrophotometer.
    • Obtain an absorbance spectrum for a molecule.
    • Use the wavelength absorption scans to determine the dyes in colored skittles.


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