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Biology LibreTexts

9: Biotechnology

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  • 9.1: Cloning and Genetic Engineering
    Nucleic acids can be isolated from cells for the purposes of further analysis by breaking open the cells and enzymatically destroying all other major macromolecules. Fragmented or whole chromosomes can be separated on the basis of size by gel electrophoresis. DNA can be cut (and subsequently re-spliced together) using restriction enzymes. The molecular and cellular techniques of biotechnology allow researchers to genetically engineer organisms, modifying them to achieve desirable traits.
  • 9.2: Biotechnology in Medicine and Agriculture
    Genetic testing is performed to identify disease-causing genes, and can be used to benefit affected individuals and their relatives who have not developed disease symptoms yet. Gene therapy—by which functioning genes are incorporated into the genomes of individuals with a non-functioning mutant gene—has the potential to cure heritable diseases. Transgenic organisms possess DNA from a different species, usually generated by molecular cloning techniques.
  • 9.3: Genomics and Proteomics
    Genome mapping is similar to solving a big, complicated puzzle with pieces of information coming from laboratories all over the world. Genetic maps provide an outline for the location of genes within a genome, and they estimate the distance between genes and genetic markers on the basis of the recombination frequency during meiosis. Physical maps provide detailed information about the physical distance between the genes. The most detailed information is available through sequence mapping.
  • 9.A: Biotechnology (Exercises)

Thumbnail: A group of Genetically modified GloFish fluorescent fish. (www.glofish.com).


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