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2: The Process of Science

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    Like other natural sciences, environmental science gathers knowledge about the natural world. Science is more than just a body of knowledge, science provides a means to evaluate and create new knowledge. The discoveries of environmental science are made by a community of researchers who work individually and together using agreed-on methods. In this sense, science is a social enterprise like politics or the arts.

    Photo A depicts round colonies of blue-green algae. Photo B depicts round fossil structures called stromatalites along a watery shoreline.
    Figure \(\PageIndex{1}\): Formerly called blue-green algae, the (a) cyanobacteria seen through a light microscope are some of Earth’s oldest life forms. These (b) stromatolites along the shores of Lake Thetis in Western Australia are ancient structures formed by the layering of cyanobacteria in shallow waters. (credit a: modification of work by NASA; scale-bar data from Matt Russell; credit b: modification of work by Ruth Ellison)

    The methods of science include careful observation, record keeping, logical and mathematical reasoning, experimentation, and submitting conclusions to the scrutiny of others. Science also requires considerable imagination and creativity; a well-designed experiment is commonly described as elegant, or beautiful. Science has considerable practical implications and some science is dedicated to practical applications, such as the prevention of disease (Figure \(\PageIndex{2}\)). Other science proceeds largely motivated by curiosity. Whatever its goal, there is no doubt that science, including biology, has transformed human existence and will continue to do so.

    Scanning electronic micrograph depicts E. coli bacteria aggregated together.
    Figure \(\PageIndex{2}\): Biologists may choose to study Escherichia coli (E. coli), a bacterium that is a normal resident of our digestive tracts but which is also sometimes responsible for disease outbreaks. In this micrograph, the bacterium is visualized using a scanning electron microscope and digital colorization. (credit: Eric Erbe; digital colorization by Christopher Pooley, USDA-ARS)

    Science is a very specific way of learning, or knowing, about the world. The history of the past 500 years demonstrates that science is a very powerful way of knowing about the world; it is largely responsible for the technological revolutions that have taken place during this time. There are however, areas of knowledge and human experience that the methods of science cannot be applied to. These include such things as answering purely moral questions, aesthetic questions, or what can be generally categorized as spiritual questions. Science has cannot investigate these areas because they are outside the realm of material phenomena, the phenomena of matter and energy, and cannot be observed and measured.

    The scientific method is a method of research with defined steps that include experiments and careful observation. The steps of the scientific method will be examined in detail later, but one of the most important aspects of this method is the testing of hypotheses. A hypothesis is a suggested explanation for an event, which can be tested. Hypotheses, or tentative explanations, are generally produced within the context of a scientific theory. A scientific theory is a generally accepted, thoroughly tested and confirmed explanation for a set of observations or phenomena. Scientific theory is the foundation of scientific knowledge. In addition, in many scientific disciplines (less so in biology) there are scientific laws, often expressed in mathematical formulas, which describe how elements of nature will behave under certain specific conditions. There is not an evolution of hypotheses through theories to laws as if they represented some increase in certainty about the world. Hypotheses are the day-to-day material that scientists work with and they are developed within the context of theories. Laws are concise descriptions of parts of the world that are amenable to formulaic or mathematical description.

    Natural Sciences

    What would you expect to see in a museum of natural sciences? Frogs? Plants? Dinosaur skeletons? Exhibits about how the brain functions? A planetarium? Gems and minerals? Or maybe all of the above? Science includes such diverse fields as astronomy, biology, computer sciences, geology, logic, physics, chemistry, and mathematics (Figure \(\PageIndex{3}\)). However, those fields of science related to the physical world and its phenomena and processes are considered natural sciences. Thus, a museum of natural sciences might contain any of the items listed above.

    Figure \(\PageIndex{3}\): Some fields of science include astronomy, biology, computer science, geology, logic, physics, chemistry, and mathematics. (credit: "Image Editor"/Flickr)

    There is no complete agreement when it comes to defining what the natural sciences include. For some experts, the natural sciences are astronomy, biology, chemistry, earth science, and physics. Other scholars choose to divide natural sciences into life sciences, which study living things and include biology, and physical sciences, which study nonliving matter and include astronomy, physics, and chemistry. Some disciplines such as biophysics and biochemistry build on two sciences and are interdisciplinary.

    Evidence, Measurements, and Observations

    Scientists use objective evidence over subjective evidence, to reach sound and logical conclusions. An objective observation is without personal bias and the same by all individuals. Bias refers to favoring one thing over another, and it can lead to inaccurate results. Humans are biased by nature, so they cannot be completely objective; the goal is to be as unbiased as possible. A subjective observation is based on a person’s feelings and beliefs and is unique to that individual (figure \(\PageIndex{4}\)).

    The waterfall is in a valley

    Figure \(\PageIndex{4}\): This is Grand Canyon of the Yellowstone in Yellowstone National Park. An objective statement about this would be, “The picture is of a waterfall.” A subjective statement would be, “The picture is beautiful.”

    Another way scientists avoid bias is by using quantitative over qualitative measurements whenever possible. A quantitative measurement is expressed with a specific numerical value. Qualitative observations are general or relative descriptions. For example, describing a rock as red or heavy is a qualitative observation. Determining a rock’s color by measuring wavelengths of reflected light or its density by measuring the proportions of minerals it contains is quantitative. Numerical values are more precise than general descriptions, and they can be analyzed using statistical calculations. This is why quantitative measurements are much more useful to scientists than qualitative observations.

    Inductive and Deductive Reasoning

    One thing is common to all forms of science: an ultimate goal “to know.” Curiosity and inquiry are the driving forces for the development of science. Scientists seek to understand the world and the way it operates. Two methods of logical thinking are used: inductive reasoning and deductive reasoning.

    Inductive reasoning is a form of logical thinking that uses related observations to arrive at a general conclusion. This type of reasoning is common in descriptive science. A life scientist such as a biologist makes observations and records them. These data can be qualitative (descriptive) or quantitative (consisting of numbers), and the raw data can be supplemented with drawings, pictures, photos, or videos. From many observations, the scientist can infer conclusions (inductions) based on evidence. Inductive reasoning involves formulating generalizations inferred from careful observation and the analysis of a large amount of data. Surveying land use (which areas are forested, agricultural, urban, etc.) across the United States and then concluding that forested areas are concentrated in the West is an example of descriptive science.

    Deductive reasoning or deduction is the type of logic used in hypothesis-based science. In deductive reasoning, the pattern of thinking moves in the opposite direction as compared to inductive reasoning. Deductive reasoning is a form of logical thinking that uses a general principle or law to forecast specific results. From those general principles, a scientist can extrapolate and predict the specific results that would be valid as long as the general principles are valid. For example, a prediction would be that if the climate is becoming warmer in a region, the distribution of plants and animals should change. Comparisons have been made between distributions in the past and the present, and the many changes that have been found are consistent with a warming climate. Finding the change in distribution is evidence that the climate change conclusion is a valid one. Deductive reasoning, or deduction, is the type of logic used in hypothesis-based science (see below).

    To summarize, inductive reasoning moves from the specific (an observation) to general (conclusion), and deductive reasoning moves from the general (a hypothesis or principle) to the specific (results).

    Both types of logical thinking are related to the two main pathways of scientific study: descriptive science and hypothesis-based science. Descriptive (or discovery) science aims to observe, explore, and discover, while hypothesis-based science begins with a specific question or problem and a potential answer or solution that can be tested. The boundary between these two forms of study is often blurred, because most scientific endeavors combine both approaches. Observations lead to questions, questions lead to forming a hypothesis as a possible answer to those questions, and then the hypothesis is tested. Thus, descriptive science and hypothesis-based science are in continuous dialogue.

    Basic and Applied Science

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    The scientific community has been debating for the last few decades about the value of different types of science. Is it valuable to pursue science for the sake of simply gaining knowledge, or does scientific knowledge only have worth if we can apply it to solving a specific problem or bettering our lives? This question focuses on the differences between two types of science: basic science and applied science.

    Basic science or “pure” science seeks to expand knowledge regardless of the short-term application of that knowledge. It is not focused on developing a product or a service of immediate public or commercial value. The immediate goal of basic science is knowledge for knowledge’s sake, though this does not mean that in the end it may not result in an application.

    In contrast, applied science or “technology,” aims to use science to solve real-world problems, making it possible, for example, to improve a crop yield, find a cure for a particular disease, or save animals threatened by a natural disaster. In applied science, the problem is usually defined for the researcher.

    Some individuals may perceive applied science as “useful” and basic science as “useless.” A question these people might pose to a scientist advocating knowledge acquisition would be, “What for?” A careful look at the history of science, however, reveals that basic knowledge has resulted in many remarkable applications of great value. Many scientists think that a basic understanding of science is necessary before an application is developed; therefore, applied science relies on the results generated through basic science. Other scientists think that it is time to move on from basic science and instead to find solutions to actual problems. Both approaches are valid. It is true that there are problems that demand immediate attention; however, few solutions would be found without the help of the knowledge generated through basic science.

    One example of how basic and applied science can work together to solve practical problems occurred after the discovery of DNA structure led to an understanding of the molecular mechanisms governing DNA replication. Strands of DNA, unique in every human, are found in our cells, where they provide the instructions necessary for life. During DNA replication, new copies of DNA are made, shortly before a cell divides to form new cells. Understanding the mechanisms of DNA replication enabled scientists to develop laboratory techniques that are now used to identify genetic diseases, pinpoint individuals who were at a crime scene, and determine paternity. Without basic science, it is unlikely that applied science would exist.

    Another example of the link between basic and applied research is the Human Genome Project, a study in which each human chromosome was analyzed and mapped to determine the precise sequence of DNA subunits and the exact location of each gene. (The gene is the basic unit of heredity; an individual’s complete collection of genes is his or her genome.) Other organisms have also been studied as part of this project to gain a better understanding of human chromosomes. The Human Genome Project (Figure 2.3.162.3.16) relied on basic research carried out with non-human organisms and, later, with the human genome. An important end goal eventually became using the data for applied research seeking cures for genetically related diseases.

    The human genome project’s logo is shown, depicting a human being inside a DNA double helix. The words chemistry, biology, physics, ethics, informatics and engineering surround the circular image.
    Figure 2.3.162.3.16: The Human Genome Project was a 13-year collaborative effort among researchers working in several different fields of science. The project was completed in 2003. (credit: the U.S. Department of Energy Genome Programs)

    While research efforts in both basic science and applied science are usually carefully planned, it is important to note that some discoveries are made by serendipity, that is, by means of a fortunate accident or a lucky surprise. Penicillin was discovered when biologist Alexander Fleming accidentally left a petri dish of Staphylococcus bacteria open. An unwanted mold grew, killing the bacteria. The mold turned out to be Penicillium, and a new antibiotic was discovered. Even in the highly organized world of science, luck—when combined with an observant, curious mind—can lead to unexpected breakthroughs.

    References

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    Neubert, D., Dillmann, I. Embryotoxic effects in mice treated with 2,4,5-trichlorophenoxyacetic acid and 2,3,7,8-tetrachlorodibenzo-p-dioxin. Naunyn-Schmiedeberg's Arch. Pharmacol. 272, 243–264 (1972).

    Stellman, J. M., & Stellman, S. D. (2018). Agent Orange During the Vietnam War: The Lingering Issue of Its Civilian and Military Health Impact. American journal of public health, 108(6), 726–728.

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    American Association for the Advancement of Science (AAAS). 1990. Science for All Americans. AAAS, Washington, DC.

    Barnes, B. 1985. About Science. Blackwell Ltd ,London, UK.

    Giere, R.N. 2005. Understanding Scientific Reasoning. 5th ed. Wadsworth Publishing, New York, NY.

    Kuhn, T.S. 1996. The Structure of Scientific Revolutions. 3rd ed. University of Chicago Press, Chicago, IL.

    McCain, G. and E.M. Siegal. 1982. The Game of Science. Holbrook Press Inc., Boston, MA.

    Moore, J.A. 1999. Science as a Way of Knowing. Harvard University Press, Boston, MA.

    Popper, K. 1979. Objective Knowledge: An Evolutionary Approach. Clarendon Press, Oxford, UK.

    Raven, P.H., G.B. Johnson, K.A. Mason, and J. Losos. 2013. Biology. 10th ed. McGraw-Hill, Columbus, OH.

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