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3.28: DNA Fingerprinting

  • Page ID
    91005
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    Preparation

    • 1X TAE buffer (enough for the electrophoresis chambers you are using and for making the gels)
    • electrophoresis gels (enough for electrophoresis chambers you are using - one for the class or one for each group)
      • 1% agarose heated in 1X TAE buffer
      • at least 3 wells are needed per group (4 if you are using a ladder)

    Materials

    • 1X TAE buffer (enough for the electrophoresis chambers you are using and for making the gels)
    • electrophoresis gels (enough for electrophoresis chambers you are using - one for the class or one for each group)
    • power supplies (enough for the electrophoresis chambers you are using)
    • DNA samples:
      • option 1: have students load loading dye into the wells, but have loading dye in different microcentrifuge tubes labeled as "East coast virus," "West coast virus," and "Midwest virus." Use this option if you don't have enough class time for letting the gel fully run and stain. If you are using this option, print out copies of one of the gel images below (1 per student) so students can analyze the results
      • option 2: purchase a virus DNA fingerprinting kit through a biology education supplier that has a quick-stain approach. You may wish to include in this option a DNA ladder.
    • sterile micropipette tips to dispense 20 μL
    • micropipettes to dispense 20 μL
    • rulers

    Gel Result Printouts

    Choose one of the gel images below if you are using option 1 described above. Print out enough of the gel of your choice for each student to have one printout.

    Midwest Virus is the East Coast Virus

    Midwest Virus Results 2

    Midwest Virus is the West Coast Virus

    Midwest Virus Results 1

    Attributions


    This page titled 3.28: DNA Fingerprinting is shared under a CC BY-NC-SA 4.0 license and was authored, remixed, and/or curated by Rosanna Hartline.

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