3.22: Nitrate Reduction
- Page ID
- 90999
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Preparation
- 24 hour cultures of Escherichia coli, Alcaligenes faecalis, and Pseudomonas aeruginosa (enough for groups to share)
- nitrate broth medium in test tubes with Durham tubes (3 per group)
Materials
- 24 hour cultures of Escherichia coli, Alcaligenes faecalis, and Pseudomonas aeruginosa (enough for groups to share)
- nitrate broth medium in test tubes with Durham tubes (3 per group)
- 0.8% sulfanilic acid in dropper bottles (enough for groups to share)
- 0.6% N, N-Dimethyl-alpha-naphthylamine (enough for groups to share)
- zinc powder (enough for groups to share)
- wooden applicator sticks for zinc powder
- test tube racks (1 per group)
- inoculating loops (1 per group)
- strikers (1 per group)
- Bunsen burners (1 per group)
- labeling tape (1 per group)
- labeling markers (1 per group)
Attributions
- Red Mountain Microbiology by Jill Raymond Ph.D.; Graham Boorse, Ph.D.; and Anne Mason M.S is licensced under CC BY-NC 4.0