# E. Metabolic Control Analysis and Systems Biology

Skills to Develop

- describe the steady state in context of an isolated single enzyme-catalyzed reaction and for the enzyme when part of a complex metabolic pathway in vivo;
- state and describe what is required to perform a metabolic control analysis on a biological pathway;
- write ordinary differential equations to describe the change in concentration of analytes in a given set of coupled reactions;
- write mass balance expressions for Eo and use it to calculate the fraction of each enzyme form assuming rapid equilibrium;
- draw "wire" diagrams showing activation and inhibition for coupled reactions;
- define the flux control coefficient verbally and in the form of a mathematic equation, and explain how to obtain it graphically;
- use flux control coefficients to explain how control of flux in a pathway is distributed through all enzyme-catalyzed reactions in that pathway;
- analyze the result of a flux control analysis of a pathway like glycolysis;
- describe why small changes in the enzymes that have the greatest ΔG values in glycolysis have minor effects in the metabolic flux in glycolysis;
- define the concentration control coefficient verbally and in the form of a mathematic equation
- analyze the result of a concentration control analysis of a pathway like glycolysis;
- define the elasticity coefficient verbally and in the form form of a mathematic equation, and explain how to obtain it graphically
- state whether the different metabolic control coefficients are global properties of the system or local properties of a given enzyme