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9.9: Protein Cleavage

Working with intact proteins in analytical techniques, such as mass spectrometry, can be problematic. Consequently, it is often desirable to break a large polypeptide down into smaller, more manageable pieces. There are two primary approaches to accomplishing this - use of chemical reagents or use of proteolytic enzymes. The table on the previous page shows the cutting specificities of various cleavage agents.

Figure 9.9.1: Protein cleaving agents