Working with intact proteins in analytical techniques, such as mass spectrometry, can be problematic. Consequently, it is often desirable to break a large polypeptide down into smaller, more manageable pieces. There are two primary approaches to accomplishing this - use of chemical reagents or use of proteolytic enzymes. The table on the previous page shows the cutting specificities of various cleavage agents.
Figure 9.9.1: Protein cleaving agents