2B: Composition, Sequence and Conformational Analysis of Proteins
- Page ID
- 4575
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Learning Objectives
describe in general terms the procedures and chemical steps in the determinations of the following for proteins:- molecular weight
- presence of certain specific amino acids
- amino acid composition
- N and C terminal amino acid
- specific amino acid necessary for binding and activity
- amino acid sequence
- secondary structure
- 3D structure
Thumbnail: Structure of human hemoglobin. The proteins α and β subunits are in red and blue, and the iron-containing heme groups in green. From PDB: 1GZX. (GNU; Proteopedia Hemoglobin).