Lactose - Lactose can be transported into E. Coli against a concentration gradient using galactoside permease, one of the proteins encoded by the lac operon. This protein uses a proton-motive force to pump lactose into the cell. The proton gradient is created by an electron transport complex in the membrane which is inhibited by cyanide, reminiscent of the cytochrome C oxidase complex in oxphos.
Figure: Lactose Transport
Glucose - Glucose can be transported into brush border cells lining the small intestine powered by a sodium-motive symport transporter.
Figure: Glucose Transport