A plaque assay, therefore, is a method by which the number of plaques observed can be used to estimate the amount of virus present in the original culture. The technique required to do so is called th...A plaque assay, therefore, is a method by which the number of plaques observed can be used to estimate the amount of virus present in the original culture. The technique required to do so is called the pour-plate technique because you will mix the virus with the bacteria with melted agar and then pour the mixture onto a plate. As we do not know the starting concentration of virus, we will need to do a serial dilution (similar to the standard plate count).
A plaque assay, therefore, is a method by which the number of plaques observed can be used to estimate the amount of virus present in the original culture. The technique required to do so is called th...A plaque assay, therefore, is a method by which the number of plaques observed can be used to estimate the amount of virus present in the original culture. The technique required to do so is called the pour-plate technique because you will mix the virus with the bacteria with melted agar and then pour the mixture onto a plate. Selective media contains ingredients that prevent the growth of unwanted microbes without inhibiting the growth of the desired microorganism.
