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- https://bio.libretexts.org/Bookshelves/Microbiology/Microbiology_(Boundless)/07%3A_Microbial_Genetics/7.22%3A_Genomics_and_Proteomics/7.22A%3A_Microarrays_and_the_TranscriptomeThe transcriptome is the set of all RNA molecules, including mRNA, rRNA, tRNA, and other non-coding RNA produced in cells.
- https://bio.libretexts.org/Bookshelves/Introductory_and_General_Biology/Map%3A_Raven_Biology_12th_Edition/17%3A_Biotechnology/17.06%3A_Medical_Applications/17.6.03%3A_MicroarraysDNA microarrays, for example, can be used to determine all of the genes that are being expressed in a given tissue, simultaneously. Microarrays employ a grid (or array) made of rows and columns on a g...DNA microarrays, for example, can be used to determine all of the genes that are being expressed in a given tissue, simultaneously. Microarrays employ a grid (or array) made of rows and columns on a glass slide, with each box of the grid containing many copies of a specific molecule, say a single-stranded DNA molecule corresponding to the sequence of a single unique gene.
- https://bio.libretexts.org/Bookshelves/Biochemistry/Book%3A_Biochemistry_Free_For_All_(Ahern_Rajagopal_and_Tan)/08%3A_Basic_Techniques/8.04%3A_Detection_identification_and_quantitation_of_specific_nucleic_acids_and_proteinsOne way to detect the presence of a particular nucleic acid or protein is dependent on transferring the separated molecules from the gels onto a membrane made of nitrocellulose or nylon to create a “b...One way to detect the presence of a particular nucleic acid or protein is dependent on transferring the separated molecules from the gels onto a membrane made of nitrocellulose or nylon to create a “blot” and probing for the molecule(s) of interest using reagents that specifically bind to those molecules. The next section will discuss how this can be done for nucleic acids as well as for proteins.
- https://bio.libretexts.org/Bookshelves/Biochemistry/Book%3A_Biochemistry_Free_and_Easy_(Ahern_and_Rajagopal)/09%3A_Techniques/9.10%3A_MicroarraysDNA microarrays, for example, can be used to determine all of the genes that are being expressed in a given tissue, simultaneously. Microarrays employ a grid (or array) made of rows and columns on a g...DNA microarrays, for example, can be used to determine all of the genes that are being expressed in a given tissue, simultaneously. Microarrays employ a grid (or array) made of rows and columns on a glass slide, with each box of the grid containing many copies of a specific molecule, say a single-stranded DNA molecule corresponding to the sequence of a single unique gene.
- https://bio.libretexts.org/Courses/Universiti_Putra_Malaysia/ESC4103_-_Environmental_Microbiology_(Universiti_Putra_Malaysia)/05%3A_Microbial_Genetics/5.11%3A_Genomics_and_Proteomics/5.11A%3A_Microarrays_and_the_TranscriptomeThe transcriptome is the set of all RNA molecules, including mRNA, rRNA, tRNA, and other non-coding RNA produced in cells.
- https://bio.libretexts.org/Bookshelves/Genetics/Working_with_Molecular_Genetics_(Hardison)/Unit_I%3A_Genes_Nucleic_Acids_Genomes_and_Chromosomes/3%3A_Isolating_and_Analyzing_Genes/3.10%3A_Functional_analysis_of_isolated_genesIdeally, the primers are in different exons so that the product of amplifying the cDNA will be smaller than the product of amplifying the genomic DNA. In complementary approaches, the labeled DNA can ...Ideally, the primers are in different exons so that the product of amplifying the cDNA will be smaller than the product of amplifying the genomic DNA. In complementary approaches, the labeled DNA can be hybridized in situto thin sections of a tissue or embryo or other specimen, and the resulting pattern of grains visualized along the specimen in the microscope (Figure 3.35).
- https://bio.libretexts.org/Bookshelves/Biochemistry/Fundamentals_of_Biochemistry_(Jakubowski_and_Flatt)/01%3A_Unit_I-_Structure_and_Catalysis/03%3A_Amino_Acids_Peptides_and_Proteins/3.5%3A_Extension_-_Antibodies_in_Quantitation_and_In_Vivo_DetectionThe page details the fundamentals of antibodies in biochemistry, focusing on their structure, function, and the techniques used to utilize them. Key areas include understanding antibody-antigen intera...The page details the fundamentals of antibodies in biochemistry, focusing on their structure, function, and the techniques used to utilize them. Key areas include understanding antibody-antigen interactions, exploring quantitation methods like ELISA, and discussing techniques like immunohistochemistry (IHC) used for detecting proteins in vivo.
