An excellent way to determine the oxygen needs of your bacterium is to grow it in different oxygen environments---atmospheric oxygen of 22%, no oxygen at all (GasPak jar), and reduced oxygen at less t...An excellent way to determine the oxygen needs of your bacterium is to grow it in different oxygen environments---atmospheric oxygen of 22%, no oxygen at all (GasPak jar), and reduced oxygen at less than 10% (candle jar)--and compare the quality and quantity of growth.