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- https://bio.libretexts.org/Courses/North_Central_State_College/BIOL_1550%3A_Microbiology_(2025)/26%3A_Laboratory_Analysis_of_the_Immune_Response/26.04%3A_Enzyme_Immunoassays_(EIA)_and_Enzyme-Linked_Immunosorbent_Assays_(ELISA)Enzyme immunoassays (EIA) are used to visualize and quantify antigens. They use an antibody conjugated to an enzyme to bind the antigen, and the enzyme converts a substrate into an observable end prod...Enzyme immunoassays (EIA) are used to visualize and quantify antigens. They use an antibody conjugated to an enzyme to bind the antigen, and the enzyme converts a substrate into an observable end product. The substrate may be either a chromogen or a fluorogen. Immunostaining is an EIA technique for visualizing cells in a tissue (immunohistochemistry) or examining intracellular structures (immunocytochemistry). Direct ELISA is used to quantify an antigen in solution.
- https://bio.libretexts.org/Bookshelves/Microbiology/Microbiology_(OpenStax)/20%3A_Laboratory_Analysis_of_the_Immune_Response/20.04%3A_Enzyme_Immunoassays_(EIA)_and_Enzyme-Linked_Immunosorbent_Assays_(ELISA)Enzyme immunoassays (EIA) are used to visualize and quantify antigens. They use an antibody conjugated to an enzyme to bind the antigen, and the enzyme converts a substrate into an observable end prod...Enzyme immunoassays (EIA) are used to visualize and quantify antigens. They use an antibody conjugated to an enzyme to bind the antigen, and the enzyme converts a substrate into an observable end product. The substrate may be either a chromogen or a fluorogen. Immunostaining is an EIA technique for visualizing cells in a tissue (immunohistochemistry) or examining intracellular structures (immunocytochemistry). Direct ELISA is used to quantify an antigen in solution.
- https://bio.libretexts.org/Courses/Sacramento_City_College/BIOL_440%3A_General_Microbiology_(Hughes)/16%3A_Week_16/27%3A_Laboratory_Analysis_of_the_Immune_Response/27.04%3A_Enzyme_Immunoassays_(EIA)_and_Enzyme-Linked_Immunosorbent_Assays_(ELISA)Enzyme immunoassays (EIA) are used to visualize and quantify antigens. They use an antibody conjugated to an enzyme to bind the antigen, and the enzyme converts a substrate into an observable end prod...Enzyme immunoassays (EIA) are used to visualize and quantify antigens. They use an antibody conjugated to an enzyme to bind the antigen, and the enzyme converts a substrate into an observable end product. The substrate may be either a chromogen or a fluorogen. Immunostaining is an EIA technique for visualizing cells in a tissue (immunohistochemistry) or examining intracellular structures (immunocytochemistry). Direct ELISA is used to quantify an antigen in solution.
- https://bio.libretexts.org/Courses/City_College_of_San_Francisco/Introduction_to_Microbiology_(Liu_et_al.)/26%3A_Diagnosing_Infections/26.05%3A_Enzyme_Immunoassays_(EIA)_and_Enzyme-Linked_Immunosorbent_Assays_(ELISA)This page details various immunoassay techniques, emphasizing enzyme immunoassays (EIAs) and enzyme-linked immunosorbent assays (ELISAs) for antigen and antibody detection. It covers different ELISA m...This page details various immunoassay techniques, emphasizing enzyme immunoassays (EIAs) and enzyme-linked immunosorbent assays (ELISAs) for antigen and antibody detection. It covers different ELISA methods (direct, indirect, sandwich) and other techniques such as immunohistochemistry. Additionally, it addresses MMR titer tests for healthcare workers' immunity verification.
