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- https://bio.libretexts.org/Bookshelves/Biochemistry/Supplemental_Modules_(Biochemistry)/2%3A_Bacteria/2.5%3A_Gene_and_OperonThe entire nucleic acid sequence that is necessary for the synthesis of a functional polypeptide or RNA molecule. Thus, a gene contains additional sequence information beyond that which codes for the ...The entire nucleic acid sequence that is necessary for the synthesis of a functional polypeptide or RNA molecule. Thus, a gene contains additional sequence information beyond that which codes for the amino acids in a protein or the nucleotides in an RNA molecule. The gene also contains the DNA necessary to get a particular transcript made.
- https://bio.libretexts.org/Bookshelves/Introductory_and_General_Biology/Biology_(Kimball)/09%3A_Regulation_of_Gene_Expression/9.13%3A_RibozymesThis page discusses ribozymes, RNA molecules with catalytic properties, which were discovered two decades ago, shifting the focus from proteins as the sole enzymes. It highlights their role in RNA pro...This page discusses ribozymes, RNA molecules with catalytic properties, which were discovered two decades ago, shifting the focus from proteins as the sole enzymes. It highlights their role in RNA processing, particularly in tRNA, rRNA, and mRNA, often through self-splicing. Key examples include ribonuclease P and Group I and II introns. Additionally, it touches on spliceosomes and viroids, noting that viroids can infect plants and exhibit self-splicing capabilities similar to ribozymes.
- https://bio.libretexts.org/Bookshelves/Genetics/Working_with_Molecular_Genetics_(Hardison)/Unit_I%3A_Genes_Nucleic_Acids_Genomes_and_Chromosomes/3%3A_Isolating_and_Analyzing_Genes/3.08%3A_Eukaryotic_gene_structureMuch can be learned about any gene after it has been isolated by recombinant DNA techniques. The structure of coding and noncoding regions, the DNA sequence, and more can be deduced. This is true for ...Much can be learned about any gene after it has been isolated by recombinant DNA techniques. The structure of coding and noncoding regions, the DNA sequence, and more can be deduced. This is true for bacterial and viral genes, as well as eukaryotic cellular genes. The next sections of this chapter will focus on analysis of eukaryotic genes, showing the power of examining purified copies of genes.
- https://bio.libretexts.org/Bookshelves/Genetics/Working_with_Molecular_Genetics_(Hardison)/Unit_I%3A_Genes_Nucleic_Acids_Genomes_and_Chromosomes/3%3A_Isolating_and_Analyzing_Genes/3.09%3A_Introns_and_ExonsFar more exons and introns have been discovered (or more accurately, predicted) throught the analysis of genomic DNA sequences than could ever be discovered by direct experimentation. The different ty...Far more exons and introns have been discovered (or more accurately, predicted) throught the analysis of genomic DNA sequences than could ever be discovered by direct experimentation. The different types of exons, the enormous length of introns, and other factors have complicated the task of finding reliable diagnostic signatures for exons in genomic sequences. However, considerable progress has been made and continues in current research.
- https://bio.libretexts.org/Bookshelves/Genetics/Working_with_Molecular_Genetics_(Hardison)/Unit_III%3A_The_Pathway_of_Gene_Expression/12%3A_RNA_processing/12.3%3A_Multiple_Mechanisms_are_Used_for_Splicing_Different_Types_of_IntronsAt least four distinct classes of introns have been identified: Introns in nuclear protein-coding genes that are removed by spliceosomes (spliceosomal introns) Introns in nuclear and archaeal transfer...At least four distinct classes of introns have been identified: Introns in nuclear protein-coding genes that are removed by spliceosomes (spliceosomal introns) Introns in nuclear and archaeal transfer RNA genes that are removed by proteins (tRNA introns) Self-splicing group I introns that are removed by RNA catalysis.
- https://bio.libretexts.org/Bookshelves/Introductory_and_General_Biology/Biology_(Kimball)/06%3A_Gene_Expression/6.06%3A_Expressed_Sequence_TagsThis page discusses vertebrate DNA, emphasizing that only a small percentage encodes proteins, while much consists of non-coding regions. Identifying genes is complex, even with complete genomic data....This page discusses vertebrate DNA, emphasizing that only a small percentage encodes proteins, while much consists of non-coding regions. Identifying genes is complex, even with complete genomic data. Transcriptome analysis, which examines mRNA from various cell types, aids in gene identification. Expressed Sequence Tags (ESTs) are used to determine gene activity by isolating mRNA, converting it to cDNA, and sequencing it for genomic matches.
