The Kirby-Bauer test for antibiotic susceptibility (also called the disc diffusion test) is a standard that has been used for years. It has been superseded by automated tests, but the K-B is still use...The Kirby-Bauer test for antibiotic susceptibility (also called the disc diffusion test) is a standard that has been used for years. It has been superseded by automated tests, but the K-B is still used in some labs, or used with certain bacteria that automation does not work well with. This test is used to determine the resistance or sensitivity of aerobes or facultative anaerobes to specific chemicals, which can then be used by the clinician for treatment of patients with bacterial infections.