The Kirby-Bauer test for antibiotic susceptibility (also called the disc diffusion test) is a standard that has been used for years. It has been superseded by automated tests, but the K-B is still use...The Kirby-Bauer test for antibiotic susceptibility (also called the disc diffusion test) is a standard that has been used for years. It has been superseded by automated tests, but the K-B is still used in some labs, or used with certain bacteria that automation does not work well with. This test is used to determine the resistance or sensitivity of aerobes or facultative anaerobes to specific chemicals, which can then be used by the clinician for treatment of patients with bacterial infections.
After completely swabbing the plate, turn it 90 degrees and repeat the swabbing process. (It is not necessary to re-moisten the swab.) Run the swab around the circumference of the plate before discard...After completely swabbing the plate, turn it 90 degrees and repeat the swabbing process. (It is not necessary to re-moisten the swab.) Run the swab around the circumference of the plate before discarding it in the discard bag. Place the metric ruler across the zone of inhibition, at the widest diameter, and measure from one edge of the zone to the other edge.
